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Crystal Structure of Moss Leafy bound to DNA

X-ray diffraction
2.32Å resolution
PDB entry 4bhk coloured by chain, front view.
PDB entry 4bhk coloured by chain, side view.
PDB entry 4bhk coloured by chain, top view.
Source organism: Physcomitrium patens
Assembly composition:
hetero tetramer (preferred)
Entry contents: 1 distinct polypeptide molecule
2 distinct DNA molecules

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Assemblies

Assembly 1 (preferred)
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Assembly Name: Floricaula/leafy-like transcription factor and DNA
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Multimeric state: hetero tetramer
Accessible surface area: 24060.1 Å2
Buried surface area: 8454.16 Å2
Dissociation area: 1,611.16 Å2
Dissociation energy (ΔGdiss): 14.71 kcal/mol
Dissociation entropy (TΔSdiss): 12.95 kcal/mol
Symmetry number: 1
PDBe Complex ID: PDB-CPX-117257

Macromolecules

Floricaula/leafy-like transcription factor
Chains: A, B
Length: 171 amino acids
Theoretical weight: 20.02 KDa
Source organism: Physcomitrium patens
Expression system: Escherichia coli
UniProt:
  • Canonical: Q94IF5 (Residues: 180-347; Coverage: 48%)
Gene names: PPLFY1, PpLFY1
Pfam: DNA Binding Domain (C-terminal) Leafy/Floricaula
InterPro:
CATH: Protein LEAFY
Floricaula/leafy-like transcription factor in PDB entry 4bhk, assembly 1, front view.
Floricaula/leafy-like transcription factor in PDB entry 4bhk, assembly 1, side view.
Floricaula/leafy-like transcription factor in PDB entry 4bhk, assembly 1, top view.
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DNA chain W
Name: MOSS-CR54 DNA
Representative chains: W
Source organism: Physcomitrium patens [3218]
Expression system: Not provided
Length: 29 nucleotides
Theoretical weight: 8.98 KDa
MOSS-CR54 DNA in PDB entry 4bhk, assembly 1, front view.
MOSS-CR54 DNA in PDB entry 4bhk, assembly 1, side view.
MOSS-CR54 DNA in PDB entry 4bhk, assembly 1, top view.
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DNA chain X
Name: MOSS-CR54 DNA
Representative chains: X
Source organism: Physcomitrium patens [3218]
Expression system: Not provided
Length: 29 nucleotides
Theoretical weight: 8.86 KDa
MOSS-CR54 DNA in PDB entry 4bhk, assembly 1, front view.
MOSS-CR54 DNA in PDB entry 4bhk, assembly 1, side view.
MOSS-CR54 DNA in PDB entry 4bhk, assembly 1, top view.
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