› 4bru

X-ray diffraction
3.24Å resolution

Crystal structure of the yeast Dhh1-Edc3 complex

Released:
DOI: 10.2210/pdb4bru/pdb
PDB entry 4bru coloured by chain, front view.
PDB entry 4bru coloured by chain, side view.
PDB entry 4bru coloured by chain, top view.
Source organism: Saccharomyces cerevisiae S288C
Primary publication:
Structural analysis of the yeast Dhh1-Pat1 complex reveals how Dhh1 engages Pat1, Edc3 and RNA in mutually exclusive interactions.
Sharif H, Ozgur S, Sharma K, Basquin C, Urlaub H, Conti E
OpenAccess logo Nucleic Acids Res 41 8377-90 (2013)
PMID: 23851565

Function and Biology Details

Reaction catalysed: 
ATP + H(2)O = ADP + phosphate
Biochemical function:
Biological process:
  • not assigned
Cellular component:
  • not assigned
Sequence domains:

Structure analysis Details

Assembly composition:
hetero dimer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-153892 (preferred)
Entry contents:
2 distinct polypeptide molecules
Macromolecules (2 distinct):
ATP-dependent RNA helicase DHH1 Chain: A
Molecule details ›
Chain: A
Length: 377 amino acids
Theoretical weight: 42.84 KDa
Source organism: Saccharomyces cerevisiae S288C
Expression system: Escherichia coli BL21(DE3)
UniProt:
  • Canonical: P39517 (Residues: 46-422; Coverage: 75%)
Gene names: DHH1, YDL160C
Sequence domains:
Structure domains: P-loop containing nucleotide triphosphate hydrolases
Enhancer of mRNA-decapping protein 3 Chain: B
Molecule details ›
Chain: B
Length: 43 amino acids
Theoretical weight: 5.33 KDa
Source organism: Saccharomyces cerevisiae S288C
Expression system: Escherichia coli BL21(DE3)
UniProt:
  • Canonical: P39998 (Residues: 77-116; Coverage: 7%)
Gene names: EDC3, LSM16, YEL015W

Ligands and Environments

No bound ligands
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: SLS BEAMLINE X10SA
Spacegroup: P4122
Unit cell:
a: 105.831Å b: 105.831Å c: 124.648Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.21 0.208 0.246
Expression system: Escherichia coli BL21(DE3)

Quick links

Citations

15 review citations

Polysomes, Stress Granules, and Processing Bodies: A Dynamic Triumvirate Controlling Cytoplasmic mRNA Fate and Function.
Chantarachot et al. (2018)
14 more

3 mentions without citation

The role of disordered protein regions in the assembly of decapping complexes and RNP granules.
Jonas et al. (2013)
2 more