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Pseudo-atomic model of microtubule-bound human kinesin-5 motor domain in the ADP state, based on cryo-electron microscopy experiment.

Electron Microscopy
10Å resolution
PDB entry 4ck5 coloured by chain, front view.
PDB entry 4ck5 coloured by chain, side view.
PDB entry 4ck5 coloured by chain, top view.
Source organisms:
Assembly composition:
hetero trimer (preferred)
Entry contents: 3 distinct polypeptide molecules

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Assemblies

Assembly 1 (preferred)
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Multimeric state: hetero trimer
PDBe Complex ID: PDB-CPX-156613

Macromolecules

Tubulin alpha-1D chain
Chain: A
Length: 452 amino acids
Theoretical weight: 50.24 KDa
Source organism: Bos taurus
UniProt:
  • Canonical: Q2HJ86 (Residues: 1-452; Coverage: 100%)
Gene name: TUBA1D
Pfam:
InterPro:
Tubulin alpha-1D chain in PDB entry 4ck5, assembly 1, front view.
Tubulin alpha-1D chain in PDB entry 4ck5, assembly 1, side view.
Tubulin alpha-1D chain in PDB entry 4ck5, assembly 1, top view.
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Tubulin beta-2B chain
Chain: B
Length: 445 amino acids
Theoretical weight: 49.91 KDa
Source organism: Bos taurus
UniProt:
  • Canonical: Q6B856 (Residues: 1-445; Coverage: 100%)
Gene name: TUBB2B
Pfam:
InterPro:
Tubulin beta-2B chain in PDB entry 4ck5, assembly 1, front view.
Tubulin beta-2B chain in PDB entry 4ck5, assembly 1, side view.
Tubulin beta-2B chain in PDB entry 4ck5, assembly 1, top view.
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Kinesin-like protein KIF11
Chain: C
Length: 373 amino acids
Theoretical weight: 41.67 KDa
Source organism: Homo sapiens
Expression system: Escherichia coli BL21
UniProt:
  • Canonical: P52732 (Residues: 1-367; Coverage: 35%)
Gene names: EG5, KIF11, KNSL1, TRIP5
Pfam: Kinesin motor domain
InterPro:
Kinesin-like protein KIF11 in PDB entry 4ck5, assembly 1, front view.
Kinesin-like protein KIF11 in PDB entry 4ck5, assembly 1, side view.
Kinesin-like protein KIF11 in PDB entry 4ck5, assembly 1, top view.
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