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Core UvrA/TRCF complex

X-ray diffraction
2.803Å resolution
PDB entry 4dfc coloured by chain, front view.
PDB entry 4dfc coloured by chain, side view.
PDB entry 4dfc coloured by chain, top view.
Source organism: Escherichia coli K-12
Assembly composition:
hetero dimer (preferred)
Entry contents: 2 distinct polypeptide molecules

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Assemblies

Assembly 1 (preferred)
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Assembly Name: TRCF-UvrA complex
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Multimeric state: hetero dimer
Accessible surface area: 10578.74 Å2
Buried surface area: 1749.9 Å2
Dissociation area: 874.95 Å2
Dissociation energy (ΔGdiss): -2.76 kcal/mol
Dissociation entropy (TΔSdiss): 10.73 kcal/mol
Symmetry number: 1
PDBe Complex ID: PDB-CPX-141272
Complex Portal ID: CPX-2155
Assembly 2
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Assembly Name: TRCF-UvrA complex
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Multimeric state: hetero dimer
Accessible surface area: 10655.36 Å2
Buried surface area: 1707.73 Å2
Dissociation area: 853.86 Å2
Dissociation energy (ΔGdiss): -2.4 kcal/mol
Dissociation entropy (TΔSdiss): 10.64 kcal/mol
Symmetry number: 1
PDBe Complex ID: PDB-CPX-141272
Complex Portal ID: CPX-2155

Macromolecules

Transcription-repair-coupling factor
Chains: A, C
Length: 93 amino acids
Theoretical weight: 10.58 KDa
Source organism: Escherichia coli K-12
Expression system: Escherichia coli
UniProt:
  • Canonical: P30958 (Residues: 127-213; Coverage: 8%)
Gene names: JW1100, b1114, mfd
Pfam: UvrB interaction domain
InterPro:
CATH: Penicillin-binding protein 1b domain
Transcription-repair-coupling factor in PDB entry 4dfc, assembly 1, front view.
Transcription-repair-coupling factor in PDB entry 4dfc, assembly 1, side view.
Transcription-repair-coupling factor in PDB entry 4dfc, assembly 1, top view.
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UvrABC system protein A
Chains: B, D
Length: 126 amino acids
Theoretical weight: 13.96 KDa
Source organism: Escherichia coli K-12
Expression system: Escherichia coli
UniProt:
  • Canonical: P0A698 (Residues: 131-250; Coverage: 13%)
Gene names: JW4019, b4058, dinE, uvrA
Pfam: UvrA interaction domain
InterPro: UvrA, interaction domain
CATH: Ribulose 1,5 Bisphosphate Carboxylase/Oxygenase
UvrABC system protein A in PDB entry 4dfc, assembly 1, front view.
UvrABC system protein A in PDB entry 4dfc, assembly 1, side view.
UvrABC system protein A in PDB entry 4dfc, assembly 1, top view.
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