› 4duw

X-ray diffraction
2.2Å resolution

E. coli (lacZ) beta-galactosidase (G974A) in complex with allolactose

Released:
DOI: 10.2210/pdb4duw/pdb
PDB entry 4duw coloured by chain, front view.
PDB entry 4duw coloured by chain, side view.
PDB entry 4duw coloured by chain, top view.
Source organism: Escherichia coli K-12
Primary publication:
Structural explanation for allolactose (lac operon inducer) synthesis by lacZ β-galactosidase and the evolutionary relationship between allolactose synthesis and the lac repressor.
Wheatley RW, Lo S, Jancewicz LJ, Dugdale ML, Huber RE
J Biol Chem 288 12993-3005 (2013)
PMID: 23486479

Function and Biology Details

Reaction catalysed: 
Hydrolysis of terminal non-reducing beta-D-galactose residues in beta-D-galactosides
Biochemical function:
Biological process:
Cellular component:
Sequence domains:

Structure analysis Details

Assembly composition:
homo tetramer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-133036 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecules (2 distinct):
Beta-galactosidase Chains: A, B, C, D
Molecule details ›
Chains: A, B, C, D
Length: 1052 amino acids
Theoretical weight: 119.76 KDa
Source organism: Escherichia coli K-12
Expression system: Escherichia coli
UniProt:
  • Canonical: P00722 (Residues: 10-1024; Coverage: 99%)
Gene names: JW0335, b0344, lacZ
Sequence domains:
Structure domains:

Ligands and Environments

Carbohydrate polymer : NEW Components: BGC, GAL
Carbohydrate polymer
3 bound ligands:
Ligand MG 10 x MG
Ligand NA 16 x NA
Ligand DMS 77 x DMS
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: CLSI BEAMLINE 08B1-1
Spacegroup: P212121
Unit cell:
a: 151.826Å b: 162.635Å c: 203.692Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.16 0.16 0.213
Expression system: Escherichia coli

Quick links

Citations

2 review citations

Sensing new chemicals with bacterial transcription factors.
Libis et al. (2016)
1 more

2 mentions without citation

Structural explanation for allolactose (lac operon inducer) synthesis by lacZ β-galactosidase and the evolutionary relationship between allolactose synthesis and the lac repressor.
Wheatley et al. (2013)
1 more