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E. coli (lacZ) beta-galactosidase (G974A) in complex with allolactose

X-ray diffraction
2.2Å resolution
PDB entry 4duw coloured by chain, front view.
PDB entry 4duw coloured by chain, side view.
PDB entry 4duw coloured by chain, top view.
Source organism: Escherichia coli K-12
Assembly composition:
homo tetramer (preferred)
Entry contents: 1 distinct polypeptide molecule

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Assemblies

Assembly 1 (preferred)
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Assembly Name: Beta-galactosidase
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Multimeric state: homo tetramer
Accessible surface area: 135940.8 Å2
Buried surface area: 41218.5 Å2
Dissociation area: 4,251.19 Å2
Dissociation energy (ΔGdiss): 17.24 kcal/mol
Dissociation entropy (TΔSdiss): 18.52 kcal/mol
Symmetry number: 4
PDBe Complex ID: PDB-CPX-133036

Macromolecules

Beta-galactosidase
Chains: A, B, C, D
Length: 1052 amino acids
Theoretical weight: 119.76 KDa
Source organism: Escherichia coli K-12
Expression system: Escherichia coli
UniProt:
  • Canonical: P00722 (Residues: 10-1024; Coverage: 99%)
Gene names: JW0335, b0344, lacZ
Pfam:
InterPro:
CATH:
Beta-galactosidase in PDB entry 4duw, assembly 1, front view.
Beta-galactosidase in PDB entry 4duw, assembly 1, side view.
Beta-galactosidase in PDB entry 4duw, assembly 1, top view.
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