› 4eei

X-ray diffraction
1.92Å resolution

Crystal Structure of Adenylosuccinate Lyase from Francisella tularensis Complexed with AMP and Succinate

Released:
DOI: 10.2210/pdb4eei/pdb
PDB entry 4eei coloured by chain, front view.
PDB entry 4eei coloured by chain, side view.
PDB entry 4eei coloured by chain, top view.
Source organism: Francisella tularensis subsp. tularensis SCHU S4
Entry authors: Maltseva N, Kim Y, Shatsman S, Anderson WF, Joachimiak A, Center for Structural Genomics of Infectious Diseases (CSGID)

Function and Biology Details

Reaction catalysed: 
(S)-2-(5-amino-1-(5-phospho-D-ribosyl)imidazole-4-carboxamido)succinate = fumarate + 5-amino-1-(5-phospho-D-ribosyl)imidazole-4-carboxamide
Biochemical function:
Biological process:
Cellular component:
Sequence domains:

Structure analysis Details

Assemblies composition:
homo tetramer
homo dimer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-177718 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Adenylosuccinate lyase Chains: A, B
Molecule details ›
Chains: A, B
Length: 438 amino acids
Theoretical weight: 50.18 KDa
Source organism: Francisella tularensis subsp. tularensis SCHU S4
Expression system: Escherichia coli BL21(DE3)
UniProt:
  • Canonical: Q5NIQ1 (Residues: 1-432; Coverage: 100%)
Gene names: FTT_0015, purB
Sequence domains: Lyase
Structure domains:

Ligands and Environments

5 bound ligands:
Ligand AMP 2 x AMP
Ligand EDO 17 x EDO
Ligand K 3 x K
Ligand SIN 1 x SIN
Ligand CL 2 x CL
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: APS BEAMLINE 19-ID
Spacegroup: P43212
Unit cell:
a: 88.759Å b: 88.759Å c: 254.724Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.169 0.168 0.199
Expression system: Escherichia coli BL21(DE3)

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