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Staphylococcal Nuclease double mutant I72L, I92L

X-ray diffraction
1.81Å resolution
PDB entry 4g57 coloured by chain, front view.
PDB entry 4g57 coloured by chain, side view.
PDB entry 4g57 coloured by chain, top view.
Source organism: Staphylococcus aureus
Assembly composition:
monomeric (preferred)
Entry contents: 1 distinct polypeptide molecule

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Assemblies

Assembly 1 (preferred)
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Assembly Name: Nuclease A
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Multimeric state: monomeric
Accessible surface area: 7662.73 Å2
Buried surface area: 0.0 Å2
Dissociation area: 0 Å2
Dissociation energy (ΔGdiss): 0 kcal/mol
Dissociation entropy (TΔSdiss): 0 kcal/mol
Symmetry number: 1
PDBe Complex ID: PDB-CPX-132790

Macromolecules

Nuclease A
Chain: A
Length: 135 amino acids
Theoretical weight: 15.41 KDa
Source organism: Staphylococcus aureus
Expression system: Escherichia coli
UniProt:
  • Canonical: P00644 (Residues: 89-223; Coverage: 66%)
Gene name: nuc
Pfam: Staphylococcal nuclease homologue
InterPro:
CATH: OB fold (Dihydrolipoamide Acetyltransferase, E2P)
Nuclease A in PDB entry 4g57, assembly 1, front view.
Nuclease A in PDB entry 4g57, assembly 1, side view.
Nuclease A in PDB entry 4g57, assembly 1, top view.
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