› 4i6z

X-ray diffraction
3.2Å resolution

Crystal structure of the transcriptional regulator TM1030 with 24bp DNA oligonucleotide

Released:
DOI: 10.2210/pdb4i6z/pdb
PDB entry 4i6z coloured by chain, front view.
PDB entry 4i6z coloured by chain, side view.
PDB entry 4i6z coloured by chain, top view.
Source organisms:
Entry authors: Koclega KD, Chruszcz M, Cooper DR, Petkowski JJ, Tkaczuk KL, Joachimiak A, Minor W, Midwest Center for Structural Genomics (MCSG)

Function and Biology Details

Biochemical function:
Biological process:
Cellular component:
  • not assigned
Sequence domains:

Structure analysis Details

Assembly composition:
hetero octamer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-117632 (preferred)
Entry contents:
1 distinct polypeptide molecule
1 distinct DNA molecule
Macromolecules (2 distinct):
HTH tetR-type domain-containing protein Chains: A, B
Molecule details ›
Chains: A, B
Length: 202 amino acids
Theoretical weight: 24.41 KDa
Source organism: Thermotoga maritima MSB8
Expression system: Escherichia coli BL21(DE3)
UniProt:
  • Canonical: Q9X0C0 (Residues: 1-200; Coverage: 100%)
Gene name: TM_1030
Sequence domains:
Structure domains:
DNA OLIGONUCLEOTIDE Chains: C, D
Molecule details ›
Chains: C, D
Length: 24 nucleotides
Theoretical weight: 7.37 KDa
Source organism: synthetic construct
Expression system: Not provided

Ligands and Environments

No bound ligands
1 modified residue:
Ligand MSE 16 x MSE

Experiments and Validation Details

Entry percentile scores
X-ray source: APS BEAMLINE 19-ID
Spacegroup: C2
Unit cell:
a: 187.008Å b: 45.954Å c: 82.51Å
α: 90° β: 105.6° γ: 90°
R-values:
R R work R free
0.203 0.2 0.268
Expression systems:
  • Escherichia coli BL21(DE3)
  • Not provided

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