› 4jhw

X-ray diffraction
3.6Å resolution

Crystal Structure of Respiratory Syncytial Virus Fusion Glycoprotein Stabilized in the Prefusion Conformation by Human Antibody D25

Released:
DOI: 10.2210/pdb4jhw/pdb
PDB entry 4jhw coloured by chain, front view.
PDB entry 4jhw coloured by chain, side view.
PDB entry 4jhw coloured by chain, top view.
Source organisms:
Primary publication:
Structure of RSV fusion glycoprotein trimer bound to a prefusion-specific neutralizing antibody.
McLellan JS, Chen M, Leung S, Graepel KW, Du X, Yang Y, Zhou T, Baxa U, Yasuda E, Beaumont T, Kumar A, Modjarrad K, Zheng Z, Zhao M, Xia N, Kwong PD, Graham BS
Science 340 1113-7 (2013)
PMID: 23618766

Function and Biology Details

Biochemical function:
  • not assigned
Biological process:
Cellular component:
  • not assigned
Sequence domains:

Structure analysis Details

Assembly composition:
hetero nonamer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-208926 (preferred)
Entry contents:
3 distinct polypeptide molecules
Macromolecules (3 distinct):
D25 antigen-binding fragment heavy chain Chain: H
Molecule details ›
Chain: H
Length: 230 amino acids
Theoretical weight: 24.38 KDa
Source organism: Homo sapiens
Expression system: Homo sapiens
Structure domains: Immunoglobulins
D25 light chain Chain: L
Molecule details ›
Chain: L
Length: 214 amino acids
Theoretical weight: 23.33 KDa
Source organism: Homo sapiens
Expression system: Homo sapiens
Structure domains: Immunoglobulins
Fusion glycoprotein F0 Chain: F
Molecule details ›
Chain: F
Length: 498 amino acids
Theoretical weight: 55.01 KDa
Source organism: Human respiratory syncytial virus A2
Expression system: Homo sapiens
UniProt:
  • Canonical: P03420 (Residues: 26-513; Coverage: 84%)
Gene name: F
Sequence domains: Fusion glycoprotein F0

Ligands and Environments

No bound ligands
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: APS BEAMLINE 22-ID
Spacegroup: P213
Unit cell:
a: 152.286Å b: 152.286Å c: 152.286Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.216 0.213 0.267
Expression system: Homo sapiens

Quick links

Citations

128 review citations

mRNA vaccines for infectious diseases: principles, delivery and clinical translation.
Chaudhary et al. (2021)
127 more

53 mentions without citation

Timing is everything: Fine-tuned molecular machines orchestrate paramyxovirus entry.
Bose et al. (2015)
52 more