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Pseudomonas aeruginosa MetAP T2N mutant, in Mn form

X-ray diffraction
2.2Å resolution
PDB entry 4juq coloured by chain, front view.
PDB entry 4juq coloured by chain, side view.
PDB entry 4juq coloured by chain, top view.
Source organism: Pseudomonas aeruginosa PAO1
Assemblies composition:
homo tetramer
monomeric (preferred)
Entry contents: 1 distinct polypeptide molecule

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Assemblies

Assembly 1
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Assembly Name: Methionine aminopeptidase
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Multimeric state: homo tetramer
Accessible surface area: 41975.6 Å2
Buried surface area: 3839.17 Å2
Dissociation area: 1,092.99 Å2
Dissociation energy (ΔGdiss): -29.12 kcal/mol
Dissociation entropy (TΔSdiss): 38.79 kcal/mol
Symmetry number: 1
PDBe Complex ID: PDB-CPX-191128
Assembly 2
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Assembly Name: Methionine aminopeptidase
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Multimeric state: monomeric
Accessible surface area: 11066.72 Å2
Buried surface area: 513.68 Å2
Dissociation area: 50.41 Å2
Dissociation energy (ΔGdiss): 3.35 kcal/mol
Dissociation entropy (TΔSdiss): -0.14 kcal/mol
Symmetry number: 1
PDBe Complex ID: PDB-CPX-191127
Assembly 3 (preferred)
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Assembly Name: Methionine aminopeptidase
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Multimeric state: monomeric
Accessible surface area: 10858.73 Å2
Buried surface area: 411.56 Å2
Dissociation area: 81.9 Å2
Dissociation energy (ΔGdiss): 7.51 kcal/mol
Dissociation entropy (TΔSdiss): -0.13 kcal/mol
Symmetry number: 1
PDBe Complex ID: PDB-CPX-191127
Assembly 4
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Assembly Name: Methionine aminopeptidase
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Multimeric state: monomeric
Accessible surface area: 11139.93 Å2
Buried surface area: 414.94 Å2
Dissociation area: 82.84 Å2
Dissociation energy (ΔGdiss): 7.66 kcal/mol
Dissociation entropy (TΔSdiss): -0.13 kcal/mol
Symmetry number: 1
PDBe Complex ID: PDB-CPX-191127
Assembly 5
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Assembly Name: Methionine aminopeptidase
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Multimeric state: monomeric
Accessible surface area: 10886.23 Å2
Buried surface area: 522.97 Å2
Dissociation area: 54.56 Å2
Dissociation energy (ΔGdiss): 3.36 kcal/mol
Dissociation entropy (TΔSdiss): -0.14 kcal/mol
Symmetry number: 1
PDBe Complex ID: PDB-CPX-191127

Macromolecules

Methionine aminopeptidase
Chains: A, B, C, D
Length: 280 amino acids
Theoretical weight: 31.4 KDa
Source organism: Pseudomonas aeruginosa PAO1
Expression system: Escherichia coli BL21(DE3)
UniProt:
  • Canonical: Q9HXY1 (Residues: 1-261; Coverage: 100%)
Gene names: PA3657, map
Pfam: Metallopeptidase family M24
InterPro:
CATH: Creatinase/methionine aminopeptidase superfamily
Methionine aminopeptidase in PDB entry 4juq, assembly 3, front view.
Methionine aminopeptidase in PDB entry 4juq, assembly 3, side view.
Methionine aminopeptidase in PDB entry 4juq, assembly 3, top view.
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