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Structure of Mtb RNAP Beta subunit B1 and B2 domains

X-ray diffraction
2.453Å resolution
PDB entry 4kbj coloured by chain, front view.
PDB entry 4kbj coloured by chain, side view.
PDB entry 4kbj coloured by chain, top view.
Source organism: Mycobacterium tuberculosis
Assembly composition:
homo dimer (preferred)
Entry contents: 1 distinct polypeptide molecule

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Assemblies

Assembly 1 (preferred)
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Assembly Name: DNA-directed RNA polymerase subunit beta
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Multimeric state: homo dimer
Accessible surface area: 36508.31 Å2
Buried surface area: 2187.74 Å2
Dissociation area: 1,093.87 Å2
Dissociation energy (ΔGdiss): 7.71 kcal/mol
Dissociation entropy (TΔSdiss): 13.84 kcal/mol
Symmetry number: 1
PDBe Complex ID: PDB-CPX-161532

Macromolecules

DNA-directed RNA polymerase subunit beta
Chains: A, B
Length: 409 amino acids
Theoretical weight: 46.27 KDa
Source organism: Mycobacterium tuberculosis
Expression system: Escherichia coli
UniProt:
  • Canonical: P9WGY9 (Residues: 53-439; Coverage: 33%)
Gene names: MTCI376.08c, Rv0667, rpoB
Pfam:
InterPro:
CATH: RNA polymerase Rpb2, domain 2
DNA-directed RNA polymerase subunit beta in PDB entry 4kbj, assembly 1, front view.
DNA-directed RNA polymerase subunit beta in PDB entry 4kbj, assembly 1, side view.
DNA-directed RNA polymerase subunit beta in PDB entry 4kbj, assembly 1, top view.
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