› 4kl5

X-ray diffraction
1.72Å resolution

Crystal structure of NpuDnaE intein

Released:
DOI: 10.2210/pdb4kl5/pdb
PDB entry 4kl5 coloured by chain, front view.
PDB entry 4kl5 coloured by chain, side view.
PDB entry 4kl5 coloured by chain, top view.
Source organism: Nostoc punctiforme PCC 73102
Primary publication:
Intermolecular domain swapping induces intein-mediated protein alternative splicing.
Aranko AS, Oeemig JS, Kajander T, Iwaï H
Nat Chem Biol 9 616-22 (2013)
PMID: 23974115

Function and Biology Details

Reaction catalysed: 
Deoxynucleoside triphosphate + DNA(n) = diphosphate + DNA(n+1)
Biochemical function:
  • not assigned
Biological process:
Cellular component:
  • not assigned
Sequence domains:

Structure analysis Details

Assemblies composition:
monomeric
homo dimer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-502423 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
DNA polymerase III, alpha subunit; Nucleic acid binding, OB-fold, tRNA/helicase-type Chains: A, B
Molecule details ›
Chains: A, B
Length: 144 amino acids
Theoretical weight: 16.38 KDa
Source organism: Nostoc punctiforme PCC 73102
Expression system: Escherichia coli
UniProt:
  • Canonical: B2J066 (Residues: 775-876; Coverage: 12%)
  • Canonical: B2J821 (Residues: 2-40; Coverage: 9%)
Gene names: Npun_F4872, Npun_F5684
Structure domains: Hedgehog/Intein (Hint) domain

Ligands and Environments

2 bound ligands:
Ligand CIT 2 x CIT
Ligand GOL 1 x GOL
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: ESRF BEAMLINE ID23-1
Spacegroup: P212121
Unit cell:
a: 57.555Å b: 66.7Å c: 67.486Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.171 0.169 0.208
Expression system: Escherichia coli

Quick links

Citations

6 review citations

Nature's recipe for splitting inteins.
Aranko et al. (2014)
5 more

5 mentions without citation

Chemoenzymatic Semisynthesis of Proteins.
Thompson et al. (2020)
4 more