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Crystal structure of a putative CpaE2 pilus assembly protein (CpaE2) from Sinorhizobium meliloti 1021 at 2.72 A resolution (PSI Community Target, Shapiro)

X-ray diffraction
2.72Å resolution
PDB entry 4myr coloured by chain, front view.
PDB entry 4myr coloured by chain, side view.
PDB entry 4myr coloured by chain, top view.
Source organism: Sinorhizobium meliloti 1021
Assembly composition:
monomeric (preferred)
Entry contents: 1 distinct polypeptide molecule

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Assemblies

Assembly 1 (preferred)
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Assembly Name: CpaE2 pilus assembly protein
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Multimeric state: monomeric
Accessible surface area: 6810.14 Å2
Buried surface area: 0.0 Å2
Dissociation area: 0 Å2
Dissociation energy (ΔGdiss): 0 kcal/mol
Dissociation entropy (TΔSdiss): 0 kcal/mol
Symmetry number: 1
PDBe Complex ID: PDB-CPX-187961
Assembly 2
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Assembly Name: CpaE2 pilus assembly protein
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Multimeric state: monomeric
Accessible surface area: 6897.1 Å2
Buried surface area: 0.0 Å2
Dissociation area: 0 Å2
Dissociation energy (ΔGdiss): 0 kcal/mol
Dissociation entropy (TΔSdiss): 0 kcal/mol
Symmetry number: 1
PDBe Complex ID: PDB-CPX-187961
Assembly 3
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Assembly Name: CpaE2 pilus assembly protein
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Multimeric state: monomeric
Accessible surface area: 6882.61 Å2
Buried surface area: 0.0 Å2
Dissociation area: 0 Å2
Dissociation energy (ΔGdiss): 0 kcal/mol
Dissociation entropy (TΔSdiss): 0 kcal/mol
Symmetry number: 1
PDBe Complex ID: PDB-CPX-187961
Assembly 4
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Assembly Name: CpaE2 pilus assembly protein
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Multimeric state: monomeric
Accessible surface area: 6709.34 Å2
Buried surface area: 0.0 Å2
Dissociation area: 0 Å2
Dissociation energy (ΔGdiss): 0 kcal/mol
Dissociation entropy (TΔSdiss): 0 kcal/mol
Symmetry number: 1
PDBe Complex ID: PDB-CPX-187961

Macromolecules

CpaE2 pilus assembly protein
Chains: A, B, C, D
Length: 147 amino acids
Theoretical weight: 16.32 KDa
Source organism: Sinorhizobium meliloti 1021
Expression system: Escherichia coli
UniProt:
  • Canonical: Q92YL8 (Residues: 30-157; Coverage: 22%)
Gene names: SMa1573, cpaE2
InterPro: CheY-like superfamily
CATH: Rossmann fold
CpaE2 pilus assembly protein in PDB entry 4myr, assembly 1, front view.
CpaE2 pilus assembly protein in PDB entry 4myr, assembly 1, side view.
CpaE2 pilus assembly protein in PDB entry 4myr, assembly 1, top view.
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