› 4neg

X-ray diffraction
2.2Å resolution

The crystal structure of tryptophan synthase subunit beta from Bacillus anthracis str. 'Ames Ancestor'

Released:
DOI: 10.2210/pdb4neg/pdb
PDB entry 4neg coloured by chain, front view.
PDB entry 4neg coloured by chain, side view.
PDB entry 4neg coloured by chain, top view.
Source organism: Bacillus anthracis str. 'Ames Ancestor'
Entry authors: Tan K, Zhang R, Zhou M, Kwon K, Anderson WF, Joachimiak A, Center for Structural Genomics of Infectious Diseases (CSGID)

Function and Biology Details

Reaction catalysed: 
(1a) 1-C-(indol-3-yl)glycerol 3-phosphate = indole + D-glyceraldehyde 3-phosphate
Biochemical function:
Biological process:
Cellular component:
  • not assigned
Sequence domains:

Structure analysis Details

Assembly composition:
homo dimer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-182598 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Tryptophan synthase beta chain Chains: A, B
Molecule details ›
Chains: A, B
Length: 400 amino acids
Theoretical weight: 44.57 KDa
Source organism: Bacillus anthracis str. 'Ames Ancestor'
Expression system: Escherichia coli BL21(DE3)
UniProt:
  • Canonical: Q81TL8 (Residues: 1-397; Coverage: 100%)
Gene names: BAS1161, BA_1253, GBAA_1253, trpB
Sequence domains: Pyridoxal-phosphate dependent enzyme
Structure domains: Rossmann fold

Ligands and Environments

3 bound ligands:
Ligand SO4 3 x SO4
Ligand FMT 2 x FMT
Ligand GOL 1 x GOL
1 modified residue:
Ligand MSE 28 x MSE

Experiments and Validation Details

Entry percentile scores
X-ray source: APS BEAMLINE 19-ID
Spacegroup: C2
Unit cell:
a: 200.762Å b: 49.037Å c: 76.494Å
α: 90° β: 99.35° γ: 90°
R-values:
R R work R free
0.177 0.175 0.217
Expression system: Escherichia coli BL21(DE3)

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