4njj

X-ray diffraction
2.7Å resolution

Crystal Structure of QueE from Burkholderia multivorans in complex with AdoMet, 6-carboxy-5,6,7,8-tetrahydropterin, and Manganese(II)

Released:

Function and Biology Details

Reaction catalysed:
6-carboxy-5,6,7,8-tetrahydropterin = 7-carboxy-7-carbaguanine + NH(3)
Biochemical function:
Biological process:
Cellular component:
  • not assigned

Structure analysis Details

Assembly composition:
homo dimer (preferred)
PDBe Complex ID:
PDB-CPX-102048 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
7-carboxy-7-deazaguanine synthase Chains: A, B
Molecule details ›
Chains: A, B
Length: 230 amino acids
Theoretical weight: 25.34 KDa
Source organism: Burkholderia multivorans ATCC 17616
Expression system: Escherichia coli
UniProt:
  • Canonical: A0A0H3KB22 (Residues: 1-210; Coverage: 100%)
Gene names: BMULJ_00116, Bmul_3115, queE
Structure domains: Aldolase class I

Ligands and Environments


Cofactor: Ligand SAM 2 x SAM
3 bound ligands:
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: APS BEAMLINE 24-ID-E
Spacegroup: P43212
Unit cell:
a: 119.174Å b: 119.174Å c: 105.31Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.163 0.161 0.2
Expression system: Escherichia coli