› 4npk

X-ray diffraction
2.55Å resolution

Extended-Synaptotagmin 2, C2A- and C2B-domains, calcium bound

Released:
DOI: 10.2210/pdb4npk/pdb
PDB entry 4npk coloured by chain, front view.
PDB entry 4npk coloured by chain, side view.
PDB entry 4npk coloured by chain, top view.
Source organism: Homo sapiens
Primary publication:
Structure and Ca²⁺-binding properties of the tandem C₂ domains of E-Syt2.
Xu J, Bacaj T, Zhou A, Tomchick DR, Südhof TC, Rizo J
Structure 22 269-80 (2014)
PMID: 24373768

Function and Biology Details

Biochemical function:
  • not assigned
Biological process:
Cellular component:
  • not assigned
Sequence domains:

Structure analysis Details

Assembly composition:
monomeric (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-106072 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Extended synaptotagmin-2 Chain: A
Molecule details ›
Chain: A
Length: 299 amino acids
Theoretical weight: 34.27 KDa
Source organism: Homo sapiens
Expression system: Escherichia coli BL21(DE3)
UniProt:
  • Canonical: A0FGR8 (Residues: 363-660; Coverage: 32%)
Gene names: ESYT2, FAM62B, KIAA1228
Sequence domains: C2 domain
Structure domains: C2 domain

Ligands and Environments

1 bound ligand:
Ligand CA 3 x CA
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: APS BEAMLINE 19-ID
Spacegroup: C2
Unit cell:
a: 95.84Å b: 53.194Å c: 85.276Å
α: 90° β: 99.95° γ: 90°
R-values:
R R work R free
0.225 0.222 0.286
Expression system: Escherichia coli BL21(DE3)

Quick links

Citations

7 review citations

Here, there, and everywhere: The importance of ER membrane contact sites.
Wu et al. (2018)
6 more

3 mentions without citation

SMP-domain proteins at membrane contact sites: Structure and function.
Reinisch et al. (2016)
2 more