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Crystal structure of PSLssmKate, a photoswitchable LSSmKate variant

X-ray diffraction
2.7Å resolution
PDB entry 4nws coloured by chain, front view.
PDB entry 4nws coloured by chain, side view.
PDB entry 4nws coloured by chain, top view.
Source organism: synthetic construct
Assembly composition:
homo dimer (preferred)
Entry contents: 1 distinct polypeptide molecule

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Assemblies

Assembly 1 (preferred)
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Assembly Name: Fluorescent protein FP480
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Multimeric state: homo dimer
Accessible surface area: 18251.38 Å2
Buried surface area: 4543.12 Å2
Dissociation area: 1,512.03 Å2
Dissociation energy (ΔGdiss): 14.79 kcal/mol
Dissociation entropy (TΔSdiss): 13.01 kcal/mol
Symmetry number: 2
PDBe Complex ID: PDB-CPX-112213

Macromolecules

Fluorescent protein FP480
Chains: A, B
Length: 243 amino acids
Theoretical weight: 27.62 KDa
Source organism: synthetic construct
Expression system: Escherichia coli
UniProt:
  • Canonical: D0VX33 (Residues: 1-233; Coverage: 100%)
Pfam: Green fluorescent protein
InterPro:
CATH: Green fluorescent protein
Fluorescent protein FP480 in PDB entry 4nws, assembly 1, front view.
Fluorescent protein FP480 in PDB entry 4nws, assembly 1, side view.
Fluorescent protein FP480 in PDB entry 4nws, assembly 1, top view.
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