› 4oeb

X-ray diffraction
1.85Å resolution

Structure of membrane binding protein pleurotolysin A from Pleurotus ostreatus

Released:
DOI: 10.2210/pdb4oeb/pdb
PDB entry 4oeb coloured by chain, front view.
PDB entry 4oeb coloured by chain, side view.
PDB entry 4oeb coloured by chain, top view.
Source organism: Pleurotus ostreatus
Primary publication:
Conformational changes during pore formation by the perforin-related protein pleurotolysin.
Lukoyanova N, Kondos SC, Farabella I, Law RH, Reboul CF, Caradoc-Davies TT, Spicer BA, Kleifeld O, Traore DA, Ekkel SM, Voskoboinik I, Trapani JA, Hatfaludi T, Oliver K, Hotze EM, Tweten RK, Whisstock JC, Topf M, Saibil HR, Dunstone MA
OpenAccess logo PLoS Biol 13 e1002049 (2015)
PMID: 25654333

Function and Biology Details

Biochemical function:
  • not assigned
Biological process:
Cellular component:
  • not assigned
Sequence domain:

Structure analysis Details

Assembly composition:
monomeric (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-186829 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Pleurotolysin A Chains: A, B, C, D
Molecule details ›
Chains: A, B, C, D
Length: 138 amino acids
Theoretical weight: 15.25 KDa
Source organism: Pleurotus ostreatus
Expression system: Escherichia coli
UniProt:
  • Canonical: Q8X1M9 (Residues: 1-138; Coverage: 100%)
Gene names: PlyA, plyA
Sequence domains: Aegerolysin
Structure domains: Mutm (Fpg) Protein; Chain: A, domain 2

Ligands and Environments

1 bound ligand:
Ligand SO4 4 x SO4
1 modified residue:
Ligand MSE 8 x MSE

Experiments and Validation Details

Entry percentile scores
X-ray source: AUSTRALIAN SYNCHROTRON BEAMLINE MX1
Spacegroup: P62
Unit cell:
a: 131.7Å b: 131.7Å c: 65.213Å
α: 90° β: 90° γ: 120°
R-values:
R R work R free
0.174 0.172 0.198
Expression system: Escherichia coli

Quick links

Citations

26 review citations

Pore-forming toxins: ancient, but never really out of fashion.
Dal Peraro et al. (2016)
25 more

5 mentions without citation

What Can Mushroom Proteins Teach Us about Lipid Rafts?
Grundner et al. (2021)
4 more