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Structure of bacteriophage SPN1S endolysin from Salmonella typhimurium

X-ray diffraction
1.9Å resolution
PDB entry 4ok7 coloured by chain, front view.
PDB entry 4ok7 coloured by chain, side view.
PDB entry 4ok7 coloured by chain, top view.
Source organism: Salmonella phage SPN1S
Assemblies composition:
homo trimer
monomeric (preferred)
Entry contents: 1 distinct polypeptide molecule

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Assemblies

Assembly 1
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Assembly Name: Glycoside hydrolase family 19 catalytic domain-containing protein
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Multimeric state: homo trimer
Accessible surface area: 30993.11 Å2
Buried surface area: 8623.92 Å2
Dissociation area: 3,125.38 Å2
Dissociation energy (ΔGdiss): 28.87 kcal/mol
Dissociation entropy (TΔSdiss): 26.5 kcal/mol
Symmetry number: 1
PDBe Complex ID: PDB-CPX-124743
Assembly 2 (preferred)
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Assembly Name: Glycoside hydrolase family 19 catalytic domain-containing protein
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Multimeric state: monomeric
Accessible surface area: 12545.02 Å2
Buried surface area: 785.66 Å2
Dissociation area: 68.5 Å2
Dissociation energy (ΔGdiss): -0.37 kcal/mol
Dissociation entropy (TΔSdiss): 0.88 kcal/mol
Symmetry number: 1
PDBe Complex ID: PDB-CPX-124742
Assembly 3
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Assembly Name: Glycoside hydrolase family 19 catalytic domain-containing protein
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Multimeric state: monomeric
Accessible surface area: 12475.91 Å2
Buried surface area: 944.72 Å2
Dissociation area: 57.16 Å2
Dissociation energy (ΔGdiss): -0.3 kcal/mol
Dissociation entropy (TΔSdiss): 0.87 kcal/mol
Symmetry number: 1
PDBe Complex ID: PDB-CPX-124742
Assembly 4
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Assembly Name: Glycoside hydrolase family 19 catalytic domain-containing protein
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Multimeric state: monomeric
Accessible surface area: 12208.19 Å2
Buried surface area: 657.52 Å2
Dissociation area: 0 Å2
Dissociation energy (ΔGdiss): 0 kcal/mol
Dissociation entropy (TΔSdiss): 0 kcal/mol
Symmetry number: 1
PDBe Complex ID: PDB-CPX-124742

Macromolecules

Glycoside hydrolase family 19 catalytic domain-containing protein
Chains: A, B, C
Length: 223 amino acids
Theoretical weight: 24.94 KDa
Source organism: Salmonella phage SPN1S
Expression system: Escherichia coli
UniProt:
  • Canonical: H2D0G4 (Residues: 1-208; Coverage: 100%)
Gene name: SPN1S_0028
Pfam: Chitinase class I
InterPro:
Glycoside hydrolase family 19 catalytic domain-containing protein in PDB entry 4ok7, assembly 2, front view.
Glycoside hydrolase family 19 catalytic domain-containing protein in PDB entry 4ok7, assembly 2, side view.
Glycoside hydrolase family 19 catalytic domain-containing protein in PDB entry 4ok7, assembly 2, top view.
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