› 4ore

X-ray diffraction
2.2Å resolution

Cyrstal structure of O-acetylserine sulfhydrylase ternary complex from Haemophilus influenzae at 2.2 A

Released:
DOI: 10.2210/pdb4ore/pdb
PDB entry 4ore coloured by chain, front view.
PDB entry 4ore coloured by chain, side view.
PDB entry 4ore coloured by chain, top view.
Source organism: Haemophilus influenzae Rd KW20
Entry authors: Kaushik A, Ekka MK, Singh AK, Kumaran S

Function and Biology Details

Reaction catalysed: 
O-acetyl-L-serine + hydrogen sulfide = L-cysteine + acetate
Biochemical function:
Biological process:
Cellular component:
Sequence domains:

Structure analysis Details

Assembly composition:
hetero tetramer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-155241 (preferred)
Entry contents:
2 distinct polypeptide molecules
Macromolecules (2 distinct):
Cysteine synthase Chain: X
Molecule details ›
Chain: X
Length: 333 amino acids
Theoretical weight: 35.6 KDa
Source organism: Haemophilus influenzae Rd KW20
Expression system: Escherichia coli
UniProt:
  • Canonical: P45040 (Residues: 1-316; Coverage: 100%)
Gene names: HI_1103, cysK
Sequence domains: Pyridoxal-phosphate dependent enzyme
Structure domains: Rossmann fold
Peptide inhibitor Chain: A
Molecule details ›
Chain: A
Length: 8 amino acids
Theoretical weight: 901 Da
Source organism: Haemophilus influenzae Rd KW20
Expression system: Not provided

Ligands and Environments

1 bound ligand:
Ligand OAS 1 x OAS
1 modified residue:
Ligand LLP 1 x LLP

Experiments and Validation Details

Entry percentile scores
X-ray source: RIGAKU MICROMAX-007 HF
Spacegroup: I41
Unit cell:
a: 112.22Å b: 112.22Å c: 46.03Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.226 0.223 0.279
Expression systems:
  • Escherichia coli
  • Not provided

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