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Crystal Structure of a K+ selective NaK mutant in Barium and Sodium

X-ray diffraction
1.85Å resolution
PDB entry 4pdr coloured by chain, front view.
PDB entry 4pdr coloured by chain, side view.
PDB entry 4pdr coloured by chain, top view.
Source organism: Bacillus cereus ATCC 14579
Assembly composition:
homo tetramer (preferred)
Entry contents: 1 distinct polypeptide molecule

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Assemblies

Assembly 1 (preferred)
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Assembly Name: Potassium channel domain-containing protein
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Multimeric state: homo tetramer
Accessible surface area: 18081.45 Å2
Buried surface area: 8410.99 Å2
Dissociation area: 3,652.82 Å2
Dissociation energy (ΔGdiss): 72.19 kcal/mol
Dissociation entropy (TΔSdiss): 40.26 kcal/mol
Symmetry number: 4
PDBe Complex ID: PDB-CPX-182541
Assembly 2
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Assembly Name: Potassium channel domain-containing protein
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Multimeric state: homo tetramer
Accessible surface area: 18527.59 Å2
Buried surface area: 11250.76 Å2
Dissociation area: 4,241.27 Å2
Dissociation energy (ΔGdiss): 106.32 kcal/mol
Dissociation entropy (TΔSdiss): 40.85 kcal/mol
Symmetry number: 4
PDBe Complex ID: PDB-CPX-182541

Macromolecules

Potassium channel domain-containing protein
Chains: A, B
Length: 97 amino acids
Theoretical weight: 10.77 KDa
Source organism: Bacillus cereus ATCC 14579
Expression system: Escherichia coli
UniProt:
  • Canonical: Q81HW2 (Residues: 20-110; Coverage: 80%)
Gene name: BC_0669
Pfam: Ion channel
InterPro:
CATH: Helix Hairpins
Potassium channel domain-containing protein in PDB entry 4pdr, assembly 1, front view.
Potassium channel domain-containing protein in PDB entry 4pdr, assembly 1, side view.
Potassium channel domain-containing protein in PDB entry 4pdr, assembly 1, top view.
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