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tRNA-Guanine Transglycosylase (TGT) Mutant D156N in Complex with 6-Amino-2-(methylamino)-1H,7H,8H-imidazo[4,5-g]quinazolin-8-one

X-ray diffraction
1.93Å resolution
PDB entry 4pum coloured by chain, front view.
PDB entry 4pum coloured by chain, side view.
PDB entry 4pum coloured by chain, top view.
Source organism: Zymomonas mobilis subsp. mobilis ZM4 = ATCC 31821
Assembly composition:
homo dimer (preferred)
Entry contents: 1 distinct polypeptide molecule

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Assemblies

Assembly 1 (preferred)
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Assembly Name: Queuine tRNA-ribosyltransferase
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Multimeric state: homo dimer
Accessible surface area: 25538.62 Å2
Buried surface area: 4479.21 Å2
Dissociation area: 1,718.94 Å2
Dissociation energy (ΔGdiss): 7.16 kcal/mol
Dissociation entropy (TΔSdiss): 13.99 kcal/mol
Symmetry number: 2
PDBe Complex ID: PDB-CPX-151274

Macromolecules

Queuine tRNA-ribosyltransferase
Chain: A
Length: 388 amino acids
Theoretical weight: 43.07 KDa
Source organism: Zymomonas mobilis subsp. mobilis ZM4 = ATCC 31821
Expression system: Escherichia coli BL21(DE3)
UniProt:
  • Canonical: P28720 (Residues: 1-386; Coverage: 100%)
Gene names: ZMO0363, tgt
Pfam: Queuine tRNA-ribosyltransferase
InterPro:
CATH: Queuine tRNA-ribosyltransferase-like
Queuine tRNA-ribosyltransferase in PDB entry 4pum, assembly 1, front view.
Queuine tRNA-ribosyltransferase in PDB entry 4pum, assembly 1, side view.
Queuine tRNA-ribosyltransferase in PDB entry 4pum, assembly 1, top view.
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