› 4v8t

Electron Microscopy
8.1Å resolution

Cryo-EM Structure of the 60S Ribosomal Subunit in Complex with Arx1 and Rei1

Released:
DOI: 10.2210/pdb4v8t/pdb
PDB entry 4v8t coloured by chain, front view.
PDB entry 4v8t coloured by chain, side view.
PDB entry 4v8t coloured by chain, top view.
Source organism: Saccharomyces cerevisiae S288C
Primary publication:
Cryo-EM structures of Arx1 and maturation factors Rei1 and Jjj1 bound to the 60S ribosomal subunit.
Greber BJ, Boehringer D, Montellese C, Ban N
Nat Struct Mol Biol 19 1228-33 (2012)
PMID: 23142985
Related structures: EMD-2169

Function and Biology Details

Biochemical function:
  • not assigned
Biological process:
  • not assigned
Cellular component:
  • not assigned
Sequence domains:

Structure analysis Details

Assembly composition:
hetero 50-mer (preferred)
PDBe Complex ID:
PDB-CPX-244077 (preferred)
Entry contents:
46 distinct polypeptide molecules
4 distinct RNA molecules
Macromolecules (50 distinct):
Large ribosomal subunit protein uL2A Chain: A
Molecule details ›
Chain: A
Length: 254 amino acids
Theoretical weight: 27.46 KDa
Source organism: Saccharomyces cerevisiae S288C
Large ribosomal subunit protein uL3 Chain: B
Molecule details ›
Chain: B
Length: 387 amino acids
Theoretical weight: 43.85 KDa
Source organism: Saccharomyces cerevisiae S288C
Large ribosomal subunit protein uL4A Chain: C
Large ribosomal subunit protein uL18 Chain: D
Large ribosomal subunit protein eL6A Chain: E
Large ribosomal subunit protein uL30A Chain: F
Large ribosomal subunit protein eL8A Chain: G
Large ribosomal subunit protein uL6A Chain: H
Large ribosomal subunit protein uL16 Chain: I
Large ribosomal subunit protein uL5A Chain: J
60S RIBOSOMAL PROTEIN L12 Chain: K
Large ribosomal subunit protein eL13A Chain: L
Large ribosomal subunit protein eL14A Chain: M
Large ribosomal subunit protein eL15A Chain: N
Large ribosomal subunit protein uL13A Chain: O
Large ribosomal subunit protein uL22A Chain: P
Large ribosomal subunit protein eL18A Chain: Q
Large ribosomal subunit protein eL19A Chain: R
Large ribosomal subunit protein eL20A Chain: S
Large ribosomal subunit protein eL21A Chain: T
Large ribosomal subunit protein eL22A Chain: U
Large ribosomal subunit protein uL14A Chain: V
Large ribosomal subunit protein eL24A Chain: W
Large ribosomal subunit protein uL23 Chain: X
Large ribosomal subunit protein uL24A Chain: Y
Large ribosomal subunit protein eL27A Chain: Z
Large ribosomal subunit protein uL15 Chain: a
Large ribosomal subunit protein eL29 Chain: b
Large ribosomal subunit protein eL30 Chain: c
Large ribosomal subunit protein eL31A Chain: d
Large ribosomal subunit protein eL32 Chain: e
Large ribosomal subunit protein eL33A Chain: f
Large ribosomal subunit protein eL34A Chain: g
Large ribosomal subunit protein uL29A Chain: h
Large ribosomal subunit protein eL36A Chain: i
Large ribosomal subunit protein eL37A Chain: j
Large ribosomal subunit protein eL38 Chain: k
Large ribosomal subunit protein eL39 Chain: l
Ubiquitin-ribosomal protein eL40A fusion protein Chain: m
Small ribosomal subunit protein eS32A Chain: n
Large ribosomal subunit protein eL42A Chain: o
Large ribosomal subunit protein eL43A Chain: p
Large ribosomal subunit protein uL10 Chain: q
Large ribosomal subunit protein P1A Chain: r
RIBOSOMAL PROTEIN P2 BETA Chain: s
Probable metalloprotease ARX1 Chain: t
ES27 OF THE 25S RRNA Chain: 1
25S ribosomal RNA Chain: 5
5S ribosomal RNA Chain: 7
5.8S ribosomal RNA Chain: 8

Ligands and Environments

1 bound ligand:
Ligand ZN 4 x ZN
No modified residues

Experiments and Validation Details

Entry percentile scores
Resolution: 8.1Å
Relevant EMDB volumes: EMD-2169
Expression system: Escherichia coli BL21(DE3)

Quick links

Citations

24 review citations

Ribosome biogenesis in the yeast Saccharomyces cerevisiae.
Woolford et al. (2013)
23 more

2 mentions without citation

Structure and function of the yeast listerin (Ltn1) conserved N-terminal domain in binding to stalled 60S ribosomal subunits.
Doamekpor et al. (2016)
1 more