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Crystal structure of chitinase 40 from thermophilic bacteria Streptomyces thermoviolaceus.

X-ray diffraction
2.771Å resolution
PDB entry 4w5u coloured by chain, front view.
PDB entry 4w5u coloured by chain, side view.
PDB entry 4w5u coloured by chain, top view.
Source organism: Streptomyces thermoviolaceus
Assembly composition:
monomeric (preferred)
Entry contents: 1 distinct polypeptide molecule

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Assemblies

Assembly 1 (preferred)
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Assembly Name: GH18 domain-containing protein
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Multimeric state: monomeric
Accessible surface area: 13928.72 Å2
Buried surface area: 224.17 Å2
Dissociation area: 112.09 Å2
Dissociation energy (ΔGdiss): -3.09 kcal/mol
Dissociation entropy (TΔSdiss): 1.2 kcal/mol
Symmetry number: 1
PDBe Complex ID: PDB-CPX-176321
Assembly 2
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Assembly Name: GH18 domain-containing protein
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Multimeric state: monomeric
Accessible surface area: 13767.37 Å2
Buried surface area: 239.47 Å2
Dissociation area: 119.73 Å2
Dissociation energy (ΔGdiss): -3.43 kcal/mol
Dissociation entropy (TΔSdiss): 1.2 kcal/mol
Symmetry number: 1
PDBe Complex ID: PDB-CPX-176321

Macromolecules

GH18 domain-containing protein
Chains: A, B
Length: 408 amino acids
Theoretical weight: 43.33 KDa
Source organism: Streptomyces thermoviolaceus
Expression system: Escherichia coli
UniProt:
  • Canonical: Q56077 (Residues: 31-184, 186-413; Coverage: 100%)
Gene name: chi40
Pfam: Glycosyl hydrolases family 18
InterPro:
CATH:
GH18 domain-containing protein in PDB entry 4w5u, assembly 1, front view.
GH18 domain-containing protein in PDB entry 4w5u, assembly 1, side view.
GH18 domain-containing protein in PDB entry 4w5u, assembly 1, top view.
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