› 4w97

X-ray diffraction
1.6Å resolution

Structure of ketosteroid transcriptional regulator KstR2 of Mycobacterium tuberculosis

Released:
DOI: 10.2210/pdb4w97/pdb
PDB entry 4w97 coloured by chain, front view.
PDB entry 4w97 coloured by chain, side view.
PDB entry 4w97 coloured by chain, top view.
Source organism: Mycobacterium tuberculosis H37Rv
Primary publication:
Structural and functional characterization of a ketosteroid transcriptional regulator of Mycobacterium tuberculosis.
Crowe AM, Stogios PJ, Casabon I, Evdokimova E, Savchenko A, Eltis LD
J Biol Chem 290 872-82 (2015)
PMID: 25406313

Function and Biology Details

Biochemical function:
Biological process:
Cellular component:
  • not assigned
Sequence domains:

Structure analysis Details

Assembly composition:
homo dimer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-161780 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
HTH-type transcriptional repressor KstR2 Chain: A
Molecule details ›
Chain: A
Length: 200 amino acids
Theoretical weight: 22.86 KDa
Source organism: Mycobacterium tuberculosis H37Rv
Expression system: Escherichia coli BL21(DE3)
UniProt:
  • Canonical: P9WMB9 (Residues: 2-200; Coverage: 100%)
Gene names: Rv3557c, kstR2
Sequence domains:
Structure domains:

Ligands and Environments

2 bound ligands:
Ligand UCA 1 x UCA
Ligand CL 4 x CL
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: RIGAKU MICROMAX-007 HF
Spacegroup: C2
Unit cell:
a: 72.485Å b: 90.476Å c: 49.798Å
α: 90° β: 129.68° γ: 90°
R-values:
R R work R free
0.162 0.16 0.199
Expression system: Escherichia coli BL21(DE3)

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Citations

2 review citations

Microbial degradation of steroid sex hormones: implications for environmental and ecological studies.
Chiang et al. (2020)
1 more

5 mentions without citation

Structural and functional characterization of a ketosteroid transcriptional regulator of Mycobacterium tuberculosis.
Crowe et al. (2015)
4 more