Assemblies
Assembly Name:
Protocatechuate 3,4-dioxygenase
Multimeric state:
hetero 24-mer
Accessible surface area:
170962.03 Å2
Buried surface area:
139288.02 Å2
Dissociation area:
14,730.02
Å2
Dissociation energy (ΔGdiss):
106.11
kcal/mol
Dissociation entropy (TΔSdiss):
83.72
kcal/mol
Symmetry number:
12
PDBe Complex ID:
PDB-CPX-132483
Macromolecules
Chains: A, C, E
Length: 200 amino acids
Theoretical weight: 22.28 KDa
Source organism: Pseudomonas putida
Expression system: Escherichia coli BL21(DE3)
UniProt:
Pfam: Dioxygenase
InterPro:
Length: 200 amino acids
Theoretical weight: 22.28 KDa
Source organism: Pseudomonas putida
Expression system: Escherichia coli BL21(DE3)
UniProt:
- Canonical: P00436 (Residues: 2-201; Coverage: 100%)
Pfam: Dioxygenase
InterPro:
- Intradiol ring-cleavage dioxygenase, core
- Protocatechuate 3,4-dioxygenase, alpha subunit
- Intradiol ring-cleavage dioxygenase, C-terminal
Chain: B
Length: 238 amino acids
Theoretical weight: 26.71 KDa
Source organism: Pseudomonas putida
Expression system: Escherichia coli BL21(DE3)
UniProt:
Pfam:
InterPro:
Length: 238 amino acids
Theoretical weight: 26.71 KDa
Source organism: Pseudomonas putida
Expression system: Escherichia coli BL21(DE3)
UniProt:
- Canonical: P00437 (Residues: 2-239; Coverage: 100%)
Pfam:
InterPro:
- Intradiol ring-cleavage dioxygenase, core
- Intradiol ring-cleavage dioxygenase, C-terminal
- Protocatechuate 3,4-dioxygenase, beta subunit
- Protocatechuate 3,4-dioxygenase beta subunit, N-terminal
Chains: D, F
Length: 238 amino acids
Theoretical weight: 26.7 KDa
Source organism: Pseudomonas putida
Expression system: Escherichia coli BL21(DE3)
UniProt:
Pfam:
InterPro:
Length: 238 amino acids
Theoretical weight: 26.7 KDa
Source organism: Pseudomonas putida
Expression system: Escherichia coli BL21(DE3)
UniProt:
- Canonical: P00437 (Residues: 2-239; Coverage: 100%)
Pfam:
InterPro:
- Intradiol ring-cleavage dioxygenase, core
- Intradiol ring-cleavage dioxygenase, C-terminal
- Protocatechuate 3,4-dioxygenase, beta subunit
- Protocatechuate 3,4-dioxygenase beta subunit, N-terminal