Assemblies
Assembly Name:
Protocatechuate 3,4-dioxygenase
Multimeric state:
hetero 24-mer
Accessible surface area:
166441.78 Å2
Buried surface area:
148604.24 Å2
Dissociation area:
18,315.52
Å2
Dissociation energy (ΔGdiss):
139.83
kcal/mol
Dissociation entropy (TΔSdiss):
84.66
kcal/mol
Symmetry number:
12
PDBe Complex ID:
PDB-CPX-132483
Macromolecules
Chains: A, C, E
Length: 200 amino acids
Theoretical weight: 22.28 KDa
Source organism: Pseudomonas putida
Expression system: Escherichia coli BL21(DE3)
UniProt:
Pfam: Dioxygenase
InterPro:
Length: 200 amino acids
Theoretical weight: 22.28 KDa
Source organism: Pseudomonas putida
Expression system: Escherichia coli BL21(DE3)
UniProt:
- Canonical: P00436 (Residues: 2-201; Coverage: 100%)
Pfam: Dioxygenase
InterPro:
- Intradiol ring-cleavage dioxygenase, core
- Protocatechuate 3,4-dioxygenase, alpha subunit
- Intradiol ring-cleavage dioxygenase, C-terminal
Chains: B, D
Length: 238 amino acids
Theoretical weight: 26.71 KDa
Source organism: Pseudomonas putida
Expression system: Escherichia coli BL21(DE3)
UniProt:
Pfam:
InterPro:
Length: 238 amino acids
Theoretical weight: 26.71 KDa
Source organism: Pseudomonas putida
Expression system: Escherichia coli BL21(DE3)
UniProt:
- Canonical: P00437 (Residues: 2-239; Coverage: 100%)
Pfam:
InterPro:
- Intradiol ring-cleavage dioxygenase, core
- Intradiol ring-cleavage dioxygenase, C-terminal
- Protocatechuate 3,4-dioxygenase, beta subunit
- Protocatechuate 3,4-dioxygenase beta subunit, N-terminal
Chain: F
Length: 238 amino acids
Theoretical weight: 26.73 KDa
Source organism: Pseudomonas putida
Expression system: Escherichia coli BL21(DE3)
UniProt:
Pfam:
InterPro:
Length: 238 amino acids
Theoretical weight: 26.73 KDa
Source organism: Pseudomonas putida
Expression system: Escherichia coli BL21(DE3)
UniProt:
- Canonical: P00437 (Residues: 2-239; Coverage: 100%)
Pfam:
InterPro:
- Intradiol ring-cleavage dioxygenase, core
- Intradiol ring-cleavage dioxygenase, C-terminal
- Protocatechuate 3,4-dioxygenase, beta subunit
- Protocatechuate 3,4-dioxygenase beta subunit, N-terminal