› 4wjg

X-ray diffraction
3.1Å resolution

Structure of T. brucei haptoglobin-hemoglobin receptor binding to human haptoglobin-hemoglobin

Released:
DOI: 10.2210/pdb4wjg/pdb
Model geometry
Fit model/data
PDB entry 4wjg coloured by chain, front view.
PDB entry 4wjg coloured by chain, side view.
PDB entry 4wjg coloured by chain, top view.
Source organisms:
Primary publication:
Structural basis for trypanosomal haem acquisition and susceptibility to the host innate immune system.
Stødkilde K, Torvund-Jensen M, Moestrup SK, Andersen CB
Nat Commun 5 5487 (2014)
PMID: 25410714

Function and Biology Details

Biochemical function:
Biological process:
Cellular component:
Sequence domains:

Structure analysis Details

Assembly composition:
hetero decamer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-125229 (preferred)
Entry contents:
5 distinct polypeptide molecules
Macromolecules (6 distinct):
Hemoglobin subunit alpha Chains: A, F, K, P, U, Z
Molecule details ›
Chains: A, F, K, P, U, Z
Length: 141 amino acids
Theoretical weight: 15.15 KDa
Source organism: Homo sapiens
UniProt:
  • Canonical: P69905 (Residues: 2-142; Coverage: 99%)
Gene names: HBA1, HBA2
Sequence domains: Globin
Structure domains: Globins
Hemoglobin subunit beta Chains: 1, B, G, L, Q, V
Molecule details ›
Chains: 1, B, G, L, Q, V
Length: 146 amino acids
Theoretical weight: 15.89 KDa
Source organism: Homo sapiens
UniProt:
  • Canonical: P68871 (Residues: 2-147; Coverage: 99%)
Gene name: HBB
Sequence domains: Globin
Structure domains: Globins
Haptoglobin Chains: 2, C, H, M, R, W
Iron-regulated surface determinant protein H Chains: 3, D, I, N, S, X
Haptoglobin-hemoglobin receptor Chains: 4, E, J, O, T, Y

Ligands and Environments

Carbohydrate polymer : NEW Components: NAG
Carbohydrate polymer
3 bound ligands:
Ligand HEM 12 x HEM
Ligand OXY 12 x OXY
Ligand NAG 23 x NAG
No modified residues

Experiments and Validation Details

wwPDB Validation report is not available for this entry.
X-ray source: SLS BEAMLINE X06SA
Spacegroup: P21
Unit cell:
a: 143.23Å b: 140.95Å c: 267.18Å
α: 90° β: 98.54° γ: 90°
R-values:
R R work R free
0.255 0.255 0.271
Expression systems:
  • Escherichia coli BL21(DE3)
  • Komagataella pastoris

Quick links

Citations

4 review citations

Haptoglobin.
Andersen et al. (2017)
3 more

9 mentions without citation

Iron Metabolism at the Interface between Host and Pathogen: From Nutritional Immunity to Antibacterial Development.
Marchetti et al. (2020)
8 more