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E. coli YgjD(E12A)-YeaZ heterodimer in complex with ATP

Source organism: Escherichia coli K-12
Biochemical function: identical protein binding
Biological process: maintenance of translational fidelity
Cellular component: cytosol

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EC 2.3.1.234: N(6)-L-threonylcarbamoyladenine synthase

Reaction catalysed:
L-threonylcarbamoyladenylate + adenine(37) in tRNA = AMP + N(6)-L-threonylcarbamoyladenine(37) in tRNA
Systematic name:
L-threonylcarbamoyladenylate:adenine(37) in tRNA N(6)-L-threonylcarbamoyltransferase
Alternative Name(s):
  • Kae1
  • Qri7
  • T6A synthase
  • YgjD (gene name)

GO terms

Sequence family

Pfam Protein family (Pfam)
PF00814
Domain description: tRNA N6-adenosine threonylcarbamoyltransferase
Occurring in:
  1. tRNA N6-adenosine threonylcarbamoyltransferase
  2. tRNA threonylcarbamoyladenosine biosynthesis protein TsaB
The deposited structure of PDB entry 4wq4 contains 2 copies of Pfam domain PF00814 (tRNA N6-adenosine threonylcarbamoyltransferase) in tRNA N6-adenosine threonylcarbamoyltransferase. Showing 1 copy in chain A.
The deposited structure of PDB entry 4wq4 contains 2 copies of Pfam domain PF00814 (tRNA N6-adenosine threonylcarbamoyltransferase) in tRNA threonylcarbamoyladenosine biosynthesis protein TsaB. Showing 1 copy in chain C.

InterPro InterPro annotations
IPR017861
Domain description: Kae1/TsaD family
Occurring in:
  1. tRNA N6-adenosine threonylcarbamoyltransferase
IPR000905
Domain description: Gcp-like domain
Occurring in:
  1. tRNA N6-adenosine threonylcarbamoyltransferase
  2. tRNA threonylcarbamoyladenosine biosynthesis protein TsaB
IPR043129
Domain description: ATPase, nucleotide binding domain
Occurring in:
  1. tRNA N6-adenosine threonylcarbamoyltransferase
  2. tRNA threonylcarbamoyladenosine biosynthesis protein TsaB
IPR022450
Domain description: tRNA N6-adenosine threonylcarbamoyltransferase, TsaD
Occurring in:
  1. tRNA N6-adenosine threonylcarbamoyltransferase
IPR022496
Domain description: tRNA threonylcarbamoyl adenosine modification protein TsaB
Occurring in:
  1. tRNA threonylcarbamoyladenosine biosynthesis protein TsaB
IPR017860
Domain description: Peptidase M22, conserved site
Occurring in:
  1. tRNA N6-adenosine threonylcarbamoyltransferase

Structure domain

CATH CATH domain
3.30.420.40
Class: Alpha Beta
Architecture: 2-Layer Sandwich
Topology: Nucleotidyltransferase; domain 5
Homology: Nucleotidyltransferase; domain 5
Occurring in:
  1. tRNA N6-adenosine threonylcarbamoyltransferase
  2. tRNA threonylcarbamoyladenosine biosynthesis protein TsaB
The deposited structure of PDB entry 4wq4 contains 4 copies of CATH domain 3.30.420.40 (Nucleotidyltransferase; domain 5) in tRNA N6-adenosine threonylcarbamoyltransferase. Showing 2 copies in chain A.
The deposited structure of PDB entry 4wq4 contains 4 copies of CATH domain 3.30.420.40 (Nucleotidyltransferase; domain 5) in tRNA threonylcarbamoyladenosine biosynthesis protein TsaB. Showing 2 copies in chain C.

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