› 4wwc

X-ray diffraction
2.9Å resolution

Crystal structure of full length YvoA in complex with palindromic operator DNA

Released:
DOI: 10.2210/pdb4wwc/pdb
PDB entry 4wwc coloured by chain, front view.
PDB entry 4wwc coloured by chain, side view.
PDB entry 4wwc coloured by chain, top view.
Source organism: Bacillus subtilis subsp. subtilis str. 168
Primary publication:
Structural insight into operator dre-sites recognition and effector binding in the GntR/HutC transcription regulator NagR.
Fillenberg SB, Grau FC, Seidel G, Muller YA
OpenAccess logo Nucleic Acids Res 43 1283-96 (2015)
PMID: 25564531

Function and Biology Details

Biochemical function:
Biological process:
Cellular component:
  • not assigned
Sequence domains:

Structure analysis Details

Assembly composition:
hetero tetramer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-118237 (preferred)
Entry contents:
1 distinct polypeptide molecule
1 distinct DNA molecule
Macromolecules (2 distinct):
HTH-type transcriptional repressor NagR Chains: A, B
Molecule details ›
Chains: A, B
Length: 246 amino acids
Theoretical weight: 27.82 KDa
Source organism: Bacillus subtilis subsp. subtilis str. 168
Expression system: Escherichia coli
UniProt:
  • Canonical: O34817 (Residues: 1-243; Coverage: 100%)
Gene names: BSU35030, nagR, yvoA
Sequence domains:
Structure domains:
DNA (5'-D(P*CP*AP*GP*TP*GP*GP*TP*CP*TP*AP*GP*AP*CP*CP*AP*CP*TP*GP*G)-3') Chains: E, F
Molecule details ›
Chains: E, F
Length: 19 nucleotides
Theoretical weight: 5.85 KDa
Source organism: Bacillus subtilis subsp. subtilis str. 168
Expression system: Not provided

Ligands and Environments

1 bound ligand:
Ligand EDO 7 x EDO
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: BESSY BEAMLINE 14.1
Spacegroup: P43212
Unit cell:
a: 79.979Å b: 79.979Å c: 239.963Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.234 0.23 0.279
Expression systems:
  • Escherichia coli
  • Not provided

Quick links

Citations

4 review citations

Regulation of antibiotic production in Actinobacteria: new perspectives from the post-genomic era.
van der Heul et al. (2018)
3 more

5 mentions without citation

Structural insight into operator dre-sites recognition and effector binding in the GntR/HutC transcription regulator NagR.
Fillenberg et al. (2015)
4 more