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Crystal structure of C-terminally truncated Neuronal Calcium Sensor (NCS-1) from Rattus norvegicus

X-ray diffraction
2.8Å resolution
PDB entry 4yru coloured by chain, front view.
PDB entry 4yru coloured by chain, side view.
PDB entry 4yru coloured by chain, top view.
Source organism: Rattus norvegicus
Assembly composition:
homo dimer (preferred)
Entry contents: 1 distinct polypeptide molecule

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Assemblies

Assembly 1 (preferred)
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Assembly Name: Neuronal calcium sensor 1
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Multimeric state: homo dimer
Accessible surface area: 15792.39 Å2
Buried surface area: 3916.9 Å2
Dissociation area: 1,785.18 Å2
Dissociation energy (ΔGdiss): 21.58 kcal/mol
Dissociation entropy (TΔSdiss): 12.66 kcal/mol
Symmetry number: 2
PDBe Complex ID: PDB-CPX-158588
Assembly 2
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Assembly Name: Neuronal calcium sensor 1
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Multimeric state: homo dimer
Accessible surface area: 16503.48 Å2
Buried surface area: 3568.7 Å2
Dissociation area: 1,608.55 Å2
Dissociation energy (ΔGdiss): 20.05 kcal/mol
Dissociation entropy (TΔSdiss): 12.66 kcal/mol
Symmetry number: 2
PDBe Complex ID: PDB-CPX-158588

Macromolecules

Neuronal calcium sensor 1
Chains: A, B, C, D
Length: 177 amino acids
Theoretical weight: 20.54 KDa
Source organism: Rattus norvegicus
Expression system: Escherichia coli
UniProt:
  • Canonical: P62168 (Residues: 1-177; Coverage: 93%)
Gene names: Flup, Freq, Ncs1
Pfam: EF hand
InterPro:
CATH: EF-hand
Neuronal calcium sensor 1 in PDB entry 4yru, assembly 1, front view.
Neuronal calcium sensor 1 in PDB entry 4yru, assembly 1, side view.
Neuronal calcium sensor 1 in PDB entry 4yru, assembly 1, top view.
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