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PlyCB mutant R66E

X-ray diffraction
1.72Å resolution
PDB entry 4zrz coloured by chain, front view.
PDB entry 4zrz coloured by chain, side view.
PDB entry 4zrz coloured by chain, top view.
Source organism: Streptococcus virus C1
Assembly composition:
homo octamer (preferred)
Entry contents: 1 distinct polypeptide molecule

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Assemblies

Assembly 1 (preferred)
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Assembly Name: Endolysin PlyC, small cell-wall binding subunit
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Multimeric state: homo octamer
Accessible surface area: 22467.48 Å2
Buried surface area: 12541.25 Å2
Dissociation area: 6,270.63 Å2
Dissociation energy (ΔGdiss): 58.19 kcal/mol
Dissociation entropy (TΔSdiss): 74.99 kcal/mol
Symmetry number: 8
PDBe Complex ID: PDB-CPX-182204

Macromolecules

Endolysin PlyC, small cell-wall binding subunit
Chains: A, B
Length: 71 amino acids
Theoretical weight: 7.84 KDa
Source organism: Streptococcus virus C1
Expression system: Escherichia coli BL21(DE3)
UniProt:
  • Canonical: Q7Y3F3 (Residues: 2-72; Coverage: 99%)
Gene name: orf9dod
Pfam: Streptococcus virus C1, PlyCB
CATH: Signal recognition particle alu RNA binding heterodimer, srp9/1
Nonstructural protein in PDB entry 4zrz, assembly 1, front view.
Nonstructural protein in PDB entry 4zrz, assembly 1, side view.
Nonstructural protein in PDB entry 4zrz, assembly 1, top view.
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