› 5a32

Electron Microscopy
3.44Å resolution

Electron cryo-microscopy of Cowpea Mosaic Virus containing RNA-1 (CPMVb)

Released:
DOI: 10.2210/pdb5a32/pdb
PDB entry 5a32 coloured by chain, front view.
PDB entry 5a32 coloured by chain, side view.
PDB entry 5a32 coloured by chain, top view.
Source organism: Cowpea mosaic virus
Primary publication:
Mechanisms of assembly and genome packaging in an RNA virus revealed by high-resolution cryo-EM.
Hesketh EL, Meshcheriakova Y, Dent KC, Saxena P, Thompson RF, Cockburn JJ, Lomonossoff GP, Ranson NA
Nat Commun 6 10113 (2015)
PMID: 26657148
Related structures: EMD-3013

Function and Biology Details

Biochemical function:
Biological process:
Cellular component:
Sequence domains:

Structure analysis Details

Assembly composition:
hetero 120-mer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-137124 (preferred)
Entry contents:
2 distinct polypeptide molecules
Macromolecules (2 distinct):
Mature small capsid protein Chain: A
Molecule details ›
Chain: A
Length: 189 amino acids
Theoretical weight: 20.96 KDa
Source organism: Cowpea mosaic virus
UniProt:
  • Canonical: P03599 (Residues: 834-1022; Coverage: 18%)
Sequence domains: Small coat protein
Structure domains: Jelly Rolls
Large capsid protein Chain: B
Molecule details ›
Chain: B
Length: 369 amino acids
Theoretical weight: 40.86 KDa
Source organism: Cowpea mosaic virus
UniProt:
  • Canonical: P03599 (Residues: 460-828; Coverage: 35%)
Sequence domains: Large coat protein
Structure domains: Jelly Rolls

Ligands and Environments

No bound ligands
No modified residues

Experiments and Validation Details

Entry percentile scores
Resolution: 3.44Å
Relevant EMDB volumes: EMD-3013

Quick links

Citations

13 review citations

Molecular pharming - VLPs made in plants.
Marsian et al. (2016)
12 more

4 mentions without citation

Mechanisms of assembly and genome packaging in an RNA virus revealed by high-resolution cryo-EM.
Hesketh et al. (2015)
3 more