5a32 › Citations

Mechanisms of assembly and genome packaging in an RNA virus revealed by high-resolution cryo-EM.

<div class='caption-title'>An introduction to CPMV.</div>
<div class='caption-title'>Cryo-EM structures of eVLP and CPMV-B.</div>
<div class='caption-title'>The structure of the C-terminal extension of the S subunit.</div>
Hesketh EL, Meshcheriakova Y, Dent KC, Saxena P, Thompson RF, Cockburn JJ, Lomonossoff GP, Ranson NA
OpenAccess logo Nat Commun 6 10113 (2015)
Cited: 41 times
EuropePMC logo PMID: 26657148

Abstract

Cowpea mosaic virus is a plant-infecting member of the Picornavirales and is of major interest in the development of biotechnology applications. Despite the availability of >100 crystal structures of Picornavirales capsids, relatively little is known about the mechanisms of capsid assembly and genome encapsidation. Here we have determined cryo-electron microscopy reconstructions for the wild-type virus and an empty virus-like particle, to 3.4 Å and 3.0 Å resolution, respectively, and built de novo atomic models of their capsids. These new structures reveal the C-terminal region of the small coat protein subunit, which is essential for virus assembly and which was missing from previously determined crystal structures, as well as residues that bind to the viral genome. These observations allow us to develop a new model for genome encapsidation and capsid assembly.

Articles - 5a32 mentioned but not cited (4)

  1. Mechanisms of assembly and genome packaging in an RNA virus revealed by high-resolution cryo-EM. Hesketh EL, Meshcheriakova Y, Dent KC, Saxena P, Thompson RF, Cockburn JJ, Lomonossoff GP, Ranson NA. Nat Commun 6 10113 (2015)
  2. Far-Reaching Dispersal of Borrelia burgdorferi Sensu Lato-Infected Blacklegged Ticks by Migratory Songbirds in Canada. Scott JD, Clark KL, Foley JE, Bierman BC, Durden LA. Healthcare (Basel) 6 E89 (2018)
  3. The structures of a naturally empty cowpea mosaic virus particle and its genome-containing counterpart by cryo-electron microscopy. Hesketh EL, Meshcheriakova Y, Thompson RF, Lomonossoff GP, Ranson NA. Sci Rep 7 539 (2017)
  4. Affimer reagents as tools in diagnosing plant virus diseases. Hesketh EL, Tiede C, Adamson H, Adams TL, Byrne MJ, Meshcheriakova Y, Kruse I, McPherson MJ, Lomonossoff GP, Tomlinson DC, Ranson NA. Sci Rep 9 7524 (2019)


Reviews citing this publication (13)

  1. Molecular pharming - VLPs made in plants. Marsian J, Lomonossoff GP. Curr Opin Biotechnol 37 201-206 (2016)
  2. Protein cage assembly across multiple length scales. Aumiller WM, Uchida M, Douglas T. Chem Soc Rev 47 3433-3469 (2018)
  3. Synthetic plant virology for nanobiotechnology and nanomedicine. Steele JFC, Peyret H, Saunders K, Castells-Graells R, Marsian J, Meshcheriakova Y, Lomonossoff GP. Wiley Interdiscip Rev Nanomed Nanobiotechnol 9 (2017)
  4. Cryo-electron microscopy for the study of virus assembly. Luque D, Castón JR. Nat Chem Biol 16 231-239 (2020)
  5. Electron Microscopy Methods for Virus Diagnosis and High Resolution Analysis of Viruses. Richert-Pöggeler KR, Franzke K, Hipp K, Kleespies RG. Front Microbiol 9 3255 (2018)
  6. The Roles of Picornavirus Untranslated Regions in Infection and Innate Immunity. Kloc A, Rai DK, Rieder E. Front Microbiol 9 485 (2018)
  7. Multiscale molecular dynamics simulation approaches to the structure and dynamics of viruses. Huber RG, Marzinek JK, Holdbrook DA, Bond PJ. Prog Biophys Mol Biol 128 121-132 (2017)
  8. Electron Cryomicroscopy of Viruses at Near-Atomic Resolutions. Kaelber JT, Hryc CF, Chiu W. Annu Rev Virol 4 287-308 (2017)
  9. Combining high-resolution cryo-electron microscopy and mutagenesis to develop cowpea mosaic virus for bionanotechnology. Meshcheriakova Y, Durrant A, Hesketh EL, Ranson NA, Lomonossoff GP. Biochem Soc Trans 45 1263-1269 (2017)
  10. Biological Applications at the Cutting Edge of Cryo-Electron Microscopy. Dillard RS, Hampton CM, Strauss JD, Ke Z, Altomara D, Guerrero-Ferreira RC, Kiss G, Wright ER. Microsc Microanal 24 406-419 (2018)
  11. RNA Structures and Their Role in Selective Genome Packaging. Ye L, Ambi UB, Olguin-Nava M, Gribling-Burrer AS, Ahmad S, Bohn P, Weber MM, Smyth RP. Viruses 13 1788 (2021)
  12. Computational modelling of flavivirus dynamics: The ins and outs. Huber RG, Marzinek JK, Boon PLS, Yue W, Bond PJ. Methods 185 28-38 (2021)
  13. The curious case of genome packaging and assembly in RNA viruses infecting plants. Ranjan T, Ranjan Kumar R, Ansar M, Kumar J, Mohanty A, Kumari A, Jain K, Rajani K, Dei S, Ahmad MF. Front Genet 14 1198647 (2023)

Articles citing this publication (24)

  1. An introduction to sample preparation and imaging by cryo-electron microscopy for structural biology. Thompson RF, Walker M, Siebert CA, Muench SP, Ranson NA. Methods 100 3-15 (2016)
  2. The 3.3 Å structure of a plant geminivirus using cryo-EM. Hesketh EL, Saunders K, Fisher C, Potze J, Stanley J, Lomonossoff GP, Ranson NA. Nat Commun 9 2369 (2018)
  3. New Structural Insights into the Genome and Minor Capsid Proteins of BK Polyomavirus using Cryo-Electron Microscopy. Hurdiss DL, Morgan EL, Thompson RF, Prescott EL, Panou MM, Macdonald A, Ranson NA. Structure 24 528-536 (2016)
  4. Cryo-Electron Microscopy Structure of the Macrobrachium rosenbergii Nodavirus Capsid at 7 Angstroms Resolution. Ho KL, Kueh CL, Beh PL, Tan WS, Bhella D. Sci Rep 7 2083 (2017)
  5. Cryo-Electron Microscopy Structure of Seneca Valley Virus Procapsid. Strauss M, Jayawardena N, Sun E, Easingwood RA, Burga LN, Bostina M. J Virol 92 e01927-17 (2018)
  6. Crystal Structure and Proteomics Analysis of Empty Virus-like Particles of Cowpea Mosaic Virus. Huynh NT, Hesketh EL, Saxena P, Meshcheriakova Y, Ku YC, Hoang LT, Johnson JE, Ranson NA, Lomonossoff GP, Reddy VS. Structure 24 567-575 (2016)
  7. Stability of plant virus-based nanocarriers in gastrointestinal fluids. Berardi A, Evans DJ, Baldelli Bombelli F, Lomonossoff GP. Nanoscale 10 1667-1679 (2018)
  8. Deep Sequencing of Foot-and-Mouth Disease Virus Reveals RNA Sequences Involved in Genome Packaging. Logan G, Newman J, Wright CF, Lasecka-Dykes L, Haydon DT, Cottam EM, Tuthill TJ. J Virol 92 e01159-17 (2018)
  9. Limits of variation, specific infectivity, and genome packaging of massively recoded poliovirus genomes. Song Y, Gorbatsevych O, Liu Y, Mugavero J, Shen SH, Ward CB, Asare E, Jiang P, Paul AV, Mueller S, Wimmer E. Proc Natl Acad Sci U S A 114 E8731-E8740 (2017)
  10. Combining Transient Expression and Cryo-EM to Obtain High-Resolution Structures of Luteovirid Particles. Byrne MJ, Steele JFC, Hesketh EL, Walden M, Thompson RF, Lomonossoff GP, Ranson NA. Structure 27 1761-1770.e3 (2019)
  11. Congress Foundational and Translational Research Opportunities to Improve Plant Health. Michelmore R, Coaker G, Bart R, Beattie G, Bent A, Bruce T, Cameron D, Dangl J, Dinesh-Kumar S, Edwards R, Eves-van den Akker S, Gassmann W, Greenberg JT, Hanley-Bowdoin L, Harrison RJ, Harvey J, He P, Huffaker A, Hulbert S, Innes R, Jones JDG, Kaloshian I, Kamoun S, Katagiri F, Leach J, Ma W, McDowell J, Medford J, Meyers B, Nelson R, Oliver R, Qi Y, Saunders D, Shaw M, Smart C, Subudhi P, Torrance L, Tyler B, Valent B, Walsh J. Mol Plant Microbe Interact 30 515-516 (2017)
  12. Encapsidation of Viral RNA in Picornavirales: Studies on Cowpea Mosaic Virus Demonstrate Dependence on Viral Replication. Kruse I, Peyret H, Saxena P, Lomonossoff GP. J Virol 93 e01520-18 (2019)
  13. The structure of a plant-specific partitivirus capsid reveals a unique coat protein domain architecture with an intrinsically disordered protrusion. Byrne M, Kashyap A, Esquirol L, Ranson N, Sainsbury F. Commun Biol 4 1155 (2021)
  14. Display of whole proteins on inner and outer surfaces of grapevine fanleaf virus-like particles. Belval L, Hemmer C, Sauter C, Reinbold C, Fauny JD, Berthold F, Ackerer L, Schmitt-Keichinger C, Lemaire O, Demangeat G, Ritzenthaler C. Plant Biotechnol J 14 2288-2299 (2016)
  15. Freeze-Drying To Produce Efficacious CPMV Virus-like Particles. Zheng Y, Lee PW, Wang C, Thomas LD, Stewart PL, Steinmetz NF, Pokorski JK. Nano Lett 19 2099-2105 (2019)
  16. RNA nanotechnology to build a dodecahedral genome of single-stranded RNA virus. Li H, Zhang K, Binzel DW, Shlyakhtenko LS, Lyubchenko YL, Chiu W, Guo P. RNA Biol 18 2390-2400 (2021)
  17. Amino acids at the exposed C-terminus of the S coat protein of cowpea mosaic virus play different roles in particle formation and viral systemic movement. Meshcheriakova Y, Lomonossoff GP. J Gen Virol 100 1165-1170 (2019)
  18. Primordial Capsid and Spooled ssDNA Genome Structures Unravel Ancestral Events of Eukaryotic Viruses. Munke A, Kimura K, Tomaru Y, Wang H, Yoshida K, Mito S, Hongo Y, Okamoto K. mBio 13 e0015622 (2022)
  19. Structure of Nora virus at 2.7 Å resolution and implications for receptor binding, capsid stability and taxonomy. Laurinmäki P, Shakeel S, Ekström JO, Mohammadi P, Hultmark D, Butcher SJ. Sci Rep 10 19675 (2020)
  20. Two Novel Iflaviruses Discovered in Bat Samples in Washington State. Juergens KB, Huckabee J, Greninger AL. Viruses 14 994 (2022)
  21. Transient Expression of Flavivirus Structural Proteins in Nicotiana benthamiana. Asghar N, Melik W, Paulsen KM, Pedersen BN, Bø-Granquist EG, Vikse R, Stuen S, Andersson S, Strid Å, Andreassen ÅK, Johansson M. Vaccines (Basel) 10 1667 (2022)
  22. Apple latent spherical virus structure with stable capsid frame supports quasi-stable protrusions expediting genome release. Naitow H, Hamaguchi T, Maki-Yonekura S, Isogai M, Yoshikawa N, Yonekura K. Commun Biol 3 488 (2020)
  23. Pharmacology of a Plant Virus Immunotherapy Candidate for Peritoneal Metastatic Ovarian Cancer. Omole AO, Affonso de Oliveira JF, Sutorus L, Steinmetz NF. ACS Pharmacol Transl Sci 7 445-455 (2024)
  24. Production and Purification of Virus-Like Particles by Transient Expression in Plants. Esquirol L, McNeale D, Venturi M, Sainsbury F. Methods Mol Biol 2671 387-402 (2023)


Quick links