› 5d0q

X-ray diffraction
3.5Å resolution

BamACDE complex, outer membrane beta-barrel assembly machinery (BAM) complex

Released:
DOI: 10.2210/pdb5d0q/pdb
PDB entry 5d0q coloured by chain, front view.
PDB entry 5d0q coloured by chain, side view.
PDB entry 5d0q coloured by chain, top view.
Source organism: Escherichia coli
Primary publication:
Structural basis of outer membrane protein insertion by the BAM complex.
Gu Y, Li H, Dong H, Zeng Y, Zhang Z, Paterson NG, Stansfeld PJ, Wang Z, Zhang Y, Wang W, Dong C
Nature 531 64-9 (2016)
PMID: 26901871

Function and Biology Details

Biochemical function:
  • not assigned
Biological process:
Cellular component:
Sequence domains:

Structure analysis Details

Assembly composition:
hetero tetramer (preferred)
PDBe Complex ID:
PDB-CPX-141765 (preferred)
Entry contents:
4 distinct polypeptide molecules
Macromolecules (4 distinct):
Outer membrane protein assembly factor BamA Chains: A, F
Molecule details ›
Chains: A, F
Length: 810 amino acids
Theoretical weight: 91.39 KDa
Source organism: Escherichia coli
Expression system: Escherichia coli
UniProt:
  • Canonical: P0A940 (Residues: 1-810; Coverage: 100%)
Gene names: JW0172, b0177, bamA, yaeT, yzzN, yzzY
Sequence domains:
Outer membrane protein assembly factor BamC Chains: C, G
Molecule details ›
Chains: C, G
Length: 344 amino acids
Theoretical weight: 37.3 KDa
Source organism: Escherichia coli
Expression system: Escherichia coli
UniProt:
  • Canonical: P0A903 (Residues: 1-344; Coverage: 100%)
Gene names: JW2462, b2477, bamC, dapX, nlpB
Sequence domains: NlpB/DapX lipoprotein
Outer membrane protein assembly factor BamD Chains: D, H
Molecule details ›
Chains: D, H
Length: 245 amino acids
Theoretical weight: 28.28 KDa
Source organism: Escherichia coli
Expression system: Escherichia coli
UniProt:
  • Canonical: P0AC02 (Residues: 1-245; Coverage: 100%)
Gene names: JW2577, b2595, bamD, yfiO
Sequence domains: Outer membrane lipoprotein
Structure domains: Tetratricopeptide repeat domain
Outer membrane protein assembly factor BamE Chains: E, I
Molecule details ›
Chains: E, I
Length: 123 amino acids
Theoretical weight: 13.72 KDa
Source organism: Escherichia coli
Expression system: Escherichia coli
UniProt:
  • Canonical: P0A937 (Residues: 1-113; Coverage: 100%)
Gene names: JW2598, b2617, bamE, smpA
Sequence domains: SmpA / OmlA family
Structure domains: Beta-lactamase Inhibitory Protein; Chain:B, domain 1

Ligands and Environments

No bound ligands
1 modified residue:
Ligand MSE 76 x MSE

Experiments and Validation Details

Entry percentile scores
X-ray source: DIAMOND BEAMLINE I03
Spacegroup: P42212
Unit cell:
a: 254.157Å b: 254.157Å c: 179.215Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.315 0.314 0.34
Expression system: Escherichia coli

Quick links

Citations

27 review citations

Emerging Diversity in Lipid-Protein Interactions.
Corradi et al. (2019)
26 more

29 mentions without citation

The β-barrel assembly machinery in motion.
Noinaj et al. (2017)
28 more