› 5d96

X-ray diffraction
2.3Å resolution

Oxidoreductase Fragment of Mouse QSOX1 in Complex with a FAb Fragment from an Antibody Targeting Mouse and Human QSOX1

Released:
DOI: 10.2210/pdb5d96/pdb
PDB entry 5d96 coloured by chain, front view.
PDB entry 5d96 coloured by chain, side view.
PDB entry 5d96 coloured by chain, top view.
Source organism: Mus musculus
Primary publication:
Overcoming a species-specificity barrier in development of an inhibitory antibody targeting a modulator of tumor stroma.
Grossman I, Ilani T, Fleishman SJ, Fass D
OpenAccess logo Protein Eng Des Sel 29 135-47 (2016)
PMID: 26819240

Function and Biology Details

Reaction catalysed: 
2 R'C(R)SH + O(2) = R'C(R)S-S(R)CR' + H(2)O(2)
Biochemical function:
Biological process:
  • not assigned
Cellular component:
  • not assigned
Sequence domains:

Structure analysis Details

Assembly composition:
hetero trimer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-567959 (preferred)
Entry contents:
3 distinct polypeptide molecules
Macromolecules (3 distinct):
Sulfhydryl oxidase 1 Chains: A, D
Molecule details ›
Chains: A, D
Length: 244 amino acids
Theoretical weight: 27.01 KDa
Source organism: Mus musculus
Expression system: Escherichia coli BL21
UniProt:
  • Canonical: Q8BND5 (Residues: 36-275; Coverage: 34%)
Gene names: Qscn6, Qsox1, Sox
Sequence domains:
Structure domains: Glutaredoxin
Light chain of Fab fragment from an antibody targeting mouse and human QSOX1 Chains: B, I
Molecule details ›
Chains: B, I
Length: 214 amino acids
Theoretical weight: 23.55 KDa
Source organism: Mus musculus
Expression system: Homo sapiens
Structure domains: Immunoglobulins
Heavy chain of Fab fragment from an antibody targeting mouse and human QSOX1 Chains: C, J
Molecule details ›
Chains: C, J
Length: 220 amino acids
Theoretical weight: 23.45 KDa
Source organism: Mus musculus
Expression system: Homo sapiens
Structure domains: Immunoglobulins

Ligands and Environments

No bound ligands
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: RIGAKU RUH3R
Spacegroup: P21
Unit cell:
a: 78.799Å b: 204.77Å c: 44.669Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.17 0.167 0.228
Expression systems:
  • Escherichia coli BL21
  • Homo sapiens

Quick links

Citations

3 review citations

Antibody Structure and Function: The Basis for Engineering Therapeutics.
Chiu et al. (2019)
2 more

4 mentions without citation

RosettaAntibodyDesign (RAbD): A general framework for computational antibody design.
Adolf-Bryfogle et al. (2018)
3 more