› 5dty

X-ray diffraction
1.5Å resolution

Crystal structure of rsEGFP2 in the non-fluorescent off-state

Released:
DOI: 10.2210/pdb5dty/pdb
PDB entry 5dty coloured by chain, front view.
PDB entry 5dty coloured by chain, side view.
PDB entry 5dty coloured by chain, top view.
Source organism: Aequorea victoria
Primary publication:
Rational design of ultrastable and reversibly photoswitchable fluorescent proteins for super-resolution imaging of the bacterial periplasm.
El Khatib M, Martins A, Bourgeois D, Colletier JP, Adam V
OpenAccess logo Sci Rep 6 18459 (2016)
PMID: 26732634

Function and Biology Details

Biochemical function:
Biological process:
Cellular component:
Sequence domains:

Structure analysis Details

Assembly composition:
monomeric (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-154537 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Green fluorescent protein Chain: A
Molecule details ›
Chain: A
Length: 246 amino acids
Theoretical weight: 28.1 KDa
Source organism: Aequorea victoria
Expression system: Escherichia coli
UniProt:
  • Canonical: P42212 (Residues: 1-238; Coverage: 100%)
Gene name: GFP
Sequence domains: Green fluorescent protein
Structure domains: Green fluorescent protein

Ligands and Environments

No bound ligands
1 modified residue:
Ligand PIA 1 x PIA

Experiments and Validation Details

Entry percentile scores
X-ray source: ESRF BEAMLINE ID23-2
Spacegroup: P212121
Unit cell:
a: 51.195Å b: 62.809Å c: 70.553Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.181 0.178 0.224
Expression system: Escherichia coli

Quick links

Citations

4 review citations

Bacterial Vivisection: How Fluorescence-Based Imaging Techniques Shed a Light on the Inner Workings of Bacteria.
Cambré et al. (2020)
3 more

2 mentions without citation

Rational design of ultrastable and reversibly photoswitchable fluorescent proteins for super-resolution imaging of the bacterial periplasm.
El Khatib et al. (2016)
1 more