5dty › Citations

Rational design of ultrastable and reversibly photoswitchable fluorescent proteins for super-resolution imaging of the bacterial periplasm.

<div class='caption-title'>Spectroscopic and biochemical characteristics.</div>
<div class='caption-title'>Crystal structures of rsEGFP2 and rsFolder in their on and off states.</div>
<div class='caption-title'>Cytoplasmic and periplasmic expressions and switching fatigue.</div>
El Khatib M, Martins A, Bourgeois D, Colletier JP, Adam V
OpenAccess logo Sci Rep 6 18459 (2016)
Related entries: 5dtx, 5dtz, 5du0

Cited: 29 times
EuropePMC logo PMID: 26732634

Abstract

Phototransformable fluorescent proteins are central to several nanoscopy approaches. As yet however, there is no available variant allowing super-resolution imaging in cell compartments that maintain oxidative conditions. Here, we report the rational design of two reversibly switchable fluorescent proteins able to fold and photoswitch in the bacterial periplasm, rsFolder and rsFolder2. rsFolder was designed by hybridisation of Superfolder-GFP with rsEGFP2, and inherited the fast folding properties of the former together with the rapid switching of the latter, but at the cost of a reduced switching contrast. Structural characterisation of the switching mechanisms of rsFolder and rsEGFP2 revealed different scenarios for chromophore cis-trans isomerisation and allowed designing rsFolder2, a variant of rsFolder that exhibits improved switching contrast and is amenable to RESOLFT nanoscopy. The rsFolders can be efficiently expressed in the E. coli periplasm, opening the door to the nanoscale investigation of proteins localised in hitherto non-observable cellular compartments.

Articles - 5dty mentioned but not cited (2)

  1. Rational design of ultrastable and reversibly photoswitchable fluorescent proteins for super-resolution imaging of the bacterial periplasm. El Khatib M, Martins A, Bourgeois D, Colletier JP, Adam V. Sci Rep 6 18459 (2016)
  2. Photoswitching mechanism of a fluorescent protein revealed by time-resolved crystallography and transient absorption spectroscopy. Woodhouse J, Nass Kovacs G, Coquelle N, Uriarte LM, Adam V, Barends TRM, Byrdin M, de la Mora E, Bruce Doak R, Feliks M, Field M, Fieschi F, Guillon V, Jakobs S, Joti Y, Macheboeuf P, Motomura K, Nass K, Owada S, Roome CM, Ruckebusch C, Schirò G, Shoeman RL, Thepaut M, Togashi T, Tono K, Yabashi M, Cammarata M, Foucar L, Bourgeois D, Sliwa M, Colletier JP, Schlichting I, Weik M. Nat Commun 11 741 (2020)


Reviews citing this publication (4)

  1. Bacterial Vivisection: How Fluorescence-Based Imaging Techniques Shed a Light on the Inner Workings of Bacteria. Cambré A, Aertsen A. Microbiol Mol Biol Rev 84 e00008-20 (2020)
  2. Deciphering Structural Photophysics of Fluorescent Proteins by Kinetic Crystallography. Bourgeois D. Int J Mol Sci 18 (2017)
  3. Catching a SPY: Using the SpyCatcher-SpyTag and Related Systems for Labeling and Localizing Bacterial Proteins. Hatlem D, Trunk T, Linke D, Leo JC. Int J Mol Sci 20 (2019)
  4. Photoswitchable Fluorescent Proteins: Mechanisms on Ultrafast Timescales. Tang L, Fang C. Int J Mol Sci 23 6459 (2022)

Articles citing this publication (23)

  1. Structural Evidence of Photoisomerization Pathways in Fluorescent Proteins. Chang J, Romei MG, Boxer SG. J Am Chem Soc 141 15504-15508 (2019)
  2. In vivo super-resolution RESOLFT microscopy of Drosophila melanogaster. Schnorrenberg S, Grotjohann T, Vorbrüggen G, Herzig A, Hell SW, Jakobs S. Elife 5 (2016)
  3. Chromophore twisting in the excited state of a photoswitchable fluorescent protein captured by time-resolved serial femtosecond crystallography. Coquelle N, Sliwa M, Woodhouse J, Schirò G, Adam V, Aquila A, Barends TRM, Boutet S, Byrdin M, Carbajo S, De la Mora E, Doak RB, Feliks M, Fieschi F, Foucar L, Guillon V, Hilpert M, Hunter MS, Jakobs S, Koglin JE, Kovacsova G, Lane TJ, Lévy B, Liang M, Nass K, Ridard J, Robinson JS, Roome CM, Ruckebusch C, Seaberg M, Thepaut M, Cammarata M, Demachy I, Field M, Shoeman RL, Bourgeois D, Colletier JP, Schlichting I, Weik M. Nat Chem 10 31-37 (2018)
  4. Achromatic light patterning and improved image reconstruction for parallelized RESOLFT nanoscopy. Chmyrov A, Leutenegger M, Grotjohann T, Schönle A, Keller-Findeisen J, Kastrup L, Jakobs S, Donnert G, Sahl SJ, Hell SW. Sci Rep 7 44619 (2017)
  5. Activity-Related Conformational Changes in d,d-Carboxypeptidases Revealed by In Vivo Periplasmic Förster Resonance Energy Transfer Assay in Escherichia coli. Meiresonne NY, van der Ploeg R, Hink MA, den Blaauwen T. MBio 8 (2017)
  6. Live-cell monochromatic dual-label sub-diffraction microscopy by mt-pcSOFI. Duwé S, Vandenberg W, Dedecker P. Chem. Commun. (Camb.) 53 7242-7245 (2017)
  7. First biphotochromic fluorescent protein moxSAASoti stabilized for oxidizing environment. Marynich NK, Khrenova MG, Gavshina AV, Solovyev ID, Savitsky AP. Sci Rep 12 7862 (2022)
  8. NMR Reveals Light-Induced Changes in the Dynamics of a Photoswitchable Fluorescent Protein. Christou NE, Ayala I, Giandoreggio-Barranco K, Byrdin M, Adam V, Bourgeois D, Brutscher B. Biophys J 117 2087-2100 (2019)
  9. Rational Control of Off-State Heterogeneity in a Photoswitchable Fluorescent Protein Provides Switching Contrast Enhancement. Adam V, Hadjidemetriou K, Jensen N, Shoeman RL, Woodhouse J, Aquila A, Banneville AS, Barends TRM, Bezchastnov V, Boutet S, Byrdin M, Cammarata M, Carbajo S, Eleni Christou N, Coquelle N, De la Mora E, El Khatib M, Moreno Chicano T, Bruce Doak R, Fieschi F, Foucar L, Glushonkov O, Gorel A, Grünbein ML, Hilpert M, Hunter M, Kloos M, Koglin JE, Lane TJ, Liang M, Mantovanelli A, Nass K, Nass Kovacs G, Owada S, Roome CM, Schirò G, Seaberg M, Stricker M, Thépaut M, Tono K, Ueda K, Uriarte LM, You D, Zala N, Domratcheva T, Jakobs S, Sliwa M, Schlichting I, Colletier JP, Bourgeois D, Weik M. Chemphyschem 23 e202200192 (2022)
  10. The Positive Switching Fluorescent Protein Padron2 Enables Live-Cell Reversible Saturable Optical Linear Fluorescence Transitions (RESOLFT) Nanoscopy without Sequential Illumination Steps. Konen T, Stumpf D, Grotjohann T, Jansen I, Bossi M, Weber M, Jensen N, Hell SW, Jakobs S. ACS Nano 15 9509-9521 (2021)
  11. X-ray Free Electron Laser Determination of Crystal Structures of Dark and Light States of a Reversibly Photoswitching Fluorescent Protein at Room Temperature. Hutchison CDM, Cordon-Preciado V, Morgan RML, Nakane T, Ferreira J, Dorlhiac G, Sanchez-Gonzalez A, Johnson AS, Fitzpatrick A, Fare C, Marangos JP, Yoon CH, Hunter MS, DePonte DP, Boutet S, Owada S, Tanaka R, Tono K, Iwata S, van Thor JJ. Int J Mol Sci 18 (2017)
  12. Absolute measurement of cellular activities using photochromic single-fluorophore biosensors and intermittent quantification. Bierbuesse F, Bourges AC, Gielen V, Mönkemöller V, Vandenberg W, Shen Y, Hofkens J, Berghe PV, Campbell RE, Moeyaert B, Dedecker P. Nat Commun 13 1850 (2022)
  13. Adsorption properties of BSA and DsRed proteins deposited on thin SiO2 layers: optically non-absorbing versus absorbing proteins. Scarangella A, Soumbo M, Villeneuve-Faure C, Mlayah A, Bonafos C, Monje MC, Roques C, Makasheva K. Nanotechnology 29 115101 (2018)
  14. BEST and SOFAST experiments for resonance assignment of histidine and tyrosine side chains in 13C/15N labeled proteins. Christou NE, Brutscher B. J. Biomol. NMR 72 115-124 (2018)
  15. Detection of the conformational changes of Discosoma red fluorescent proteins adhered on silver nanoparticles-based nanocomposites via surface-enhanced Raman scattering. Scarangella A, Soumbo M, Mlayah A, Bonafos C, Monje MC, Roques C, Marcelot C, Large N, Dammak T, Makasheva K. Nanotechnology 30 165101 (2019)
  16. Energetic Basis and Design of Enzyme Function Demonstrated Using GFP, an Excited-State Enzyme. Lin CY, Romei MG, Mathews II, Boxer SG. J Am Chem Soc 144 3968-3978 (2022)
  17. Extra kinetic dimensions for label discrimination. Chouket R, Pellissier-Tanon A, Lahlou A, Zhang R, Kim D, Plamont MA, Zhang M, Zhang X, Xu P, Desprat N, Bourgeois D, Espagne A, Lemarchand A, Saux TL, Jullien L. Nat Commun 13 1482 (2022)
  18. Fluorescent secreted bacterial effectors reveal active intravacuolar proliferation of Listeria monocytogenes in epithelial cells. Peron-Cane C, Fernandez JC, Leblanc J, Wingertsmann L, Gautier A, Desprat N, Lebreton A. PLoS Pathog 16 e1009001 (2020)
  19. Frame-Insensitive Expression Cloning of Fluorescent Protein from Scolionema suvaense. Horiuchi Y, Laskaratou D, Sliwa M, Ruckebusch C, Hatori K, Mizuno H, Hotta JI. Int J Mol Sci 19 (2018)
  20. Out-of-Phase Imaging after Optical Modulation (OPIOM) for Multiplexed Fluorescence Imaging Under Adverse Optical Conditions. Chouket R, Zhang R, Pellissier-Tanon A, Lemarchand A, Espagne A, Saux TL, Jullien L. Methods Mol Biol 2350 191-227 (2021)
  21. Reduced Fluorescent Protein Switching Fatigue by Binding-Induced Emissive State Stabilization. Roebroek T, Duwé S, Vandenberg W, Dedecker P. Int J Mol Sci 18 (2017)
  22. Serial Femtosecond Crystallography Reveals that Photoactivation in a Fluorescent Protein Proceeds via the Hula Twist Mechanism. Fadini A, Hutchison CDM, Morozov D, Chang J, Maghlaoui K, Perrett S, Luo F, Kho JCX, Romei MG, Morgan RML, Orr CM, Cordon-Preciado V, Fujiwara T, Nuemket N, Tosha T, Tanaka R, Owada S, Tono K, Iwata S, Boxer SG, Groenhof G, Nango E, van Thor JJ. J Am Chem Soc 145 15796-15808 (2023)
  23. Structural and spectroscopic characterization of photoactive yellow protein and photoswitchable fluorescent protein constructs containing heavy atoms. Romei MG, Lin CY, Boxer SG. J Photochem Photobiol A Chem 401 112738 (2020)


Quick links