› 5e6k

X-ray diffraction
2.16Å resolution

Ketosynthase from module 6 of the bacillaene synthase from Bacillus subtilis 168 (C167S mutant, crystal form 2)

Released:
DOI: 10.2210/pdb5e6k/pdb
PDB entry 5e6k coloured by chain, front view.
PDB entry 5e6k coloured by chain, side view.
PDB entry 5e6k coloured by chain, top view.
Source organism: Bacillus subtilis subsp. subtilis str. 168
Primary publication:
The LINKS motif zippers trans-acyltransferase polyketide synthase assembly lines into a biosynthetic megacomplex.
Gay DC, Wagner DT, Meinke JL, Zogzas CE, Gay GR, Keatinge-Clay AT
J Struct Biol 193 196-205 (2016)
PMID: 26724270

Function and Biology Details

Biochemical function:
Biological process:
  • not assigned
Cellular component:
  • not assigned
Sequence domains:

Structure analysis Details

Assembly composition:
homo dimer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-170206 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Polyketide synthase PksL Chains: A, B
Molecule details ›
Chains: A, B
Length: 617 amino acids
Theoretical weight: 69.03 KDa
Source organism: Bacillus subtilis subsp. subtilis str. 168
Expression system: Escherichia coli
UniProt:
  • Canonical: Q05470 (Residues: 2870-3466; Coverage: 13%)
Gene names: BSU17190, outG, pksA, pksL, pksX
Sequence domains:
Structure domains: Peroxisomal Thiolase; Chain A, domain 1

Ligands and Environments

1 bound ligand:
Ligand SO4 4 x SO4
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: APS BEAMLINE 23-ID-D
Spacegroup: P21
Unit cell:
a: 76.609Å b: 105.448Å c: 89.332Å
α: 90° β: 96.76° γ: 90°
R-values:
R R work R free
0.222 0.221 0.254
Expression system: Escherichia coli

Quick links

Citations

7 review citations

Evolution and Diversity of Assembly-Line Polyketide Synthases.
Nivina et al. (2019)
6 more