› 5f82

X-ray diffraction
0.96Å resolution

Apo GES-5 C69G mutant

Released:
DOI: 10.2210/pdb5f82/pdb
PDB entry 5f82 coloured by chain, front view.
PDB entry 5f82 coloured by chain, side view.
PDB entry 5f82 coloured by chain, top view.
Source organism: Pseudomonas aeruginosa
Primary publication:
Role of the Conserved Disulfide Bridge in Class A Carbapenemases.
Smith CA, Nossoni Z, Toth M, Stewart NK, Frase H, Vakulenko SB
J Biol Chem 291 22196-22206 (2016)
PMID: 27590339

Function and Biology Details

Reaction catalysed: 
A beta-lactam + H(2)O = a substituted beta-amino acid
Biochemical function:
Biological process:
Cellular component:
  • not assigned
Sequence domains:

Structure analysis Details

Assembly composition:
monomeric (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-106068 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
beta-lactamase Chains: A, B
Molecule details ›
Chains: A, B
Length: 287 amino acids
Theoretical weight: 31.18 KDa
Source organism: Pseudomonas aeruginosa
Expression system: Escherichia coli
UniProt:
  • Canonical: A0EL75 (Residues: 1-287; Coverage: 100%)
Gene name: blaGES-5
Sequence domains:
Structure domains: DD-peptidase/beta-lactamase superfamily

Ligands and Environments

1 bound ligand:
Ligand EPE 3 x EPE
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: SSRL BEAMLINE BL14-1
Spacegroup: P21
Unit cell:
a: 42.711Å b: 80.939Å c: 71.052Å
α: 90° β: 101.36° γ: 90°
R-values:
R R work R free
0.119 0.118 0.134
Expression system: Escherichia coli

Quick links

Citations

3 review citations

β-Lactamases and β-Lactamase Inhibitors in the 21st Century.
Tooke et al. (2019)
2 more

4 mentions without citation

Role of the Conserved Disulfide Bridge in Class A Carbapenemases.
Smith et al. (2016)
3 more