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Apo GES-5 C69G mutant

X-ray diffraction
0.96Å resolution
PDB entry 5f82 coloured by chain, front view.
PDB entry 5f82 coloured by chain, side view.
PDB entry 5f82 coloured by chain, top view.
Source organism: Pseudomonas aeruginosa
Assembly composition:
monomeric (preferred)
Entry contents: 1 distinct polypeptide molecule

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Assemblies

Assembly 1
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Assembly Name: beta-lactamase
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Multimeric state: monomeric
Accessible surface area: 11295.61 Å2
Buried surface area: 641.73 Å2
Dissociation area: 141.07 Å2
Dissociation energy (ΔGdiss): -6.16 kcal/mol
Dissociation entropy (TΔSdiss): 3.51 kcal/mol
Symmetry number: 1
PDBe Complex ID: PDB-CPX-106068
Assembly 2 (preferred)
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Assembly Name: beta-lactamase
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Multimeric state: monomeric
Accessible surface area: 11129.57 Å2
Buried surface area: 362.7 Å2
Dissociation area: 181.35 Å2
Dissociation energy (ΔGdiss): -5.63 kcal/mol
Dissociation entropy (TΔSdiss): 3.55 kcal/mol
Symmetry number: 1
PDBe Complex ID: PDB-CPX-106068

Macromolecules

beta-lactamase
Chains: A, B
Length: 287 amino acids
Theoretical weight: 31.18 KDa
Source organism: Pseudomonas aeruginosa
Expression system: Escherichia coli
UniProt:
  • Canonical: A0EL75 (Residues: 1-287; Coverage: 100%)
Gene name: blaGES-5
Pfam:
InterPro:
CATH: DD-peptidase/beta-lactamase superfamily
beta-lactamase in PDB entry 5f82, assembly 2, front view.
beta-lactamase in PDB entry 5f82, assembly 2, side view.
beta-lactamase in PDB entry 5f82, assembly 2, top view.
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