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S1 nuclease from Aspergillus oryzae with unoccupied active site

X-ray diffraction
1.5Å resolution
PDB entry 5fb9 coloured by chain, front view.
PDB entry 5fb9 coloured by chain, side view.
PDB entry 5fb9 coloured by chain, top view.
Source organism: Aspergillus oryzae RIB40
Assembly composition:
monomeric (preferred)
Entry contents: 1 distinct polypeptide molecule

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Assemblies

Assembly 1 (preferred)
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Assembly Name: Nuclease S1
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Multimeric state: monomeric
Accessible surface area: 11350.76 Å2
Buried surface area: 1265.64 Å2
Dissociation area: 107.24 Å2
Dissociation energy (ΔGdiss): -3.73 kcal/mol
Dissociation entropy (TΔSdiss): 2.85 kcal/mol
Symmetry number: 1
PDBe Complex ID: PDB-CPX-150145
Assembly 2
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Assembly Name: Nuclease S1
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Multimeric state: monomeric
Accessible surface area: 11156.75 Å2
Buried surface area: 1225.16 Å2
Dissociation area: 104.38 Å2
Dissociation energy (ΔGdiss): -5.2 kcal/mol
Dissociation entropy (TΔSdiss): 2.83 kcal/mol
Symmetry number: 1
PDBe Complex ID: PDB-CPX-150145

Macromolecules

Nuclease S1
Chains: A, B
Length: 267 amino acids
Theoretical weight: 29.08 KDa
Source organism: Aspergillus oryzae RIB40
Expression system: Aspergillus oryzae
UniProt:
  • Canonical: P24021 (Residues: 21-287; Coverage: 100%)
Gene names: AO090001000075, nucS
Pfam: S1/P1 Nuclease
InterPro:
CATH: P1 Nuclease
Nuclease S1 in PDB entry 5fb9, assembly 1, front view.
Nuclease S1 in PDB entry 5fb9, assembly 1, side view.
Nuclease S1 in PDB entry 5fb9, assembly 1, top view.
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