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Sulfur Transferase TtuA in complex with Sulfur Carrier TtuB

Source organism: Thermus thermophilus HB27
Biochemical function: nucleotide binding
Biological process: tRNA processing
Cellular component: cytosolic tRNA wobble base thiouridylase complex

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EC 2.8.1.15: tRNA-5-methyluridine(54) 2-sulfurtransferase

Reaction catalysed:
ATP + [TtuB sulfur-carrier protein]-Gly-NH-CH(2)-C(O)SH + 5-methyluracil(54) in tRNA + H(2)O = AMP + diphosphate + 5-methyl-2-thiouracil(54) in tRNA + [TtuB sulfur-carrier protein]-Gly-Gly
Systematic name:
[TtuB sulfur-carrier protein]-Gly-NH-CH(2)-C(O)SH:tRNA (5-methyluridine(54)-2-O)-sulfurtransferase
Alternative Name(s):
  • TtuA

GO terms

Sequence families

Pfam Protein families (Pfam)
PF02597
Domain description: ThiS family
Occurring in:
  1. Sulfur carrier protein TtuB
The deposited structure of PDB entry 5gha contains 4 copies of Pfam domain PF02597 (ThiS family) in Sulfur carrier protein TtuB. Showing 1 copy in chain G [auth F].

PF01171
Domain description: PP-loop family
Occurring in:
  1. tRNA-5-methyluridine(54) 2-sulfurtransferase
The deposited structure of PDB entry 5gha contains 4 copies of Pfam domain PF01171 (PP-loop family) in tRNA-5-methyluridine(54) 2-sulfurtransferase. Showing 1 copy in chain B [auth D].

PF22082
Domain description: 2-thiouridine synthetase TtuA, N-terminal LIM domain
Occurring in:
  1. tRNA-5-methyluridine(54) 2-sulfurtransferase

InterPro InterPro annotations
IPR014729
Domain description: Rossmann-like alpha/beta/alpha sandwich fold
Occurring in:
  1. tRNA-5-methyluridine(54) 2-sulfurtransferase
  2. Sulfur carrier protein TtuB
IPR016155
Domain description: Molybdopterin synthase/thiamin biosynthesis sulphur carrier, beta-grasp
Occurring in:
  1. Sulfur carrier protein TtuB
IPR012675
Domain description: Beta-grasp domain superfamily
Occurring in:
  1. Sulfur carrier protein TtuB
IPR000541
Domain description: Cytoplasmic tRNA 2-thiolation protein 1
Occurring in:
  1. tRNA-5-methyluridine(54) 2-sulfurtransferase
IPR011063
Domain description: tRNA(Ile)-lysidine/2-thiocytidine synthase, N-terminal
Occurring in:
  1. tRNA-5-methyluridine(54) 2-sulfurtransferase
IPR035107
Domain description: tRNA thiolation protein, TtcA/Ctu1 type
Occurring in:
  1. tRNA-5-methyluridine(54) 2-sulfurtransferase

Structure domain

CATH CATH domain
3.40.50.620
Class: Alpha Beta
Architecture: 3-Layer(aba) Sandwich
Topology: Rossmann fold
Homology: HUPs
Occurring in:
  1. tRNA-5-methyluridine(54) 2-sulfurtransferase
The deposited structure of PDB entry 5gha contains 4 copies of CATH domain 3.40.50.620 (Rossmann fold) in tRNA-5-methyluridine(54) 2-sulfurtransferase. Showing 1 copy in chain B [auth D].

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