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Sulfur Transferase TtuA in complex with Sulfur Carrier TtuB

X-ray diffraction
2.502Å resolution
PDB entry 5gha coloured by chain, front view.
PDB entry 5gha coloured by chain, side view.
PDB entry 5gha coloured by chain, top view.
Source organism: Thermus thermophilus HB27
Assembly composition:
hetero tetramer (preferred)
Entry contents: 2 distinct polypeptide molecules

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Assemblies

Assembly 1
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Assembly Name: tRNA-5-methyluridine(54) 2-sulfurtransferase and Sulfur carrier protein TtuB
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Multimeric state: hetero tetramer
Accessible surface area: 35896.17 Å2
Buried surface area: 7405.57 Å2
Dissociation area: 3,307.98 Å2
Dissociation energy (ΔGdiss): 15.97 kcal/mol
Dissociation entropy (TΔSdiss): 36.13 kcal/mol
Symmetry number: 2
PDBe Complex ID: PDB-CPX-180890
Assembly 2 (preferred)
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Assembly Name: tRNA-5-methyluridine(54) 2-sulfurtransferase and Sulfur carrier protein TtuB
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Multimeric state: hetero tetramer
Accessible surface area: 35341.6 Å2
Buried surface area: 7279.19 Å2
Dissociation area: 2,087.74 Å2
Dissociation energy (ΔGdiss): -0.17 kcal/mol
Dissociation entropy (TΔSdiss): 22.24 kcal/mol
Symmetry number: 2
PDBe Complex ID: PDB-CPX-180890

Macromolecules

tRNA-5-methyluridine(54) 2-sulfurtransferase
Chains: A, B, C, D
Length: 321 amino acids
Theoretical weight: 36.25 KDa
Source organism: Thermus thermophilus HB27
Expression system: Escherichia coli
UniProt:
  • Canonical: Q72LF3 (Residues: 1-321; Coverage: 100%)
Gene names: TT_C0106, ttuA
Pfam:
InterPro:
CATH: HUPs
tRNA-5-methyluridine(54) 2-sulfurtransferase in PDB entry 5gha, assembly 2, front view.
tRNA-5-methyluridine(54) 2-sulfurtransferase in PDB entry 5gha, assembly 2, side view.
tRNA-5-methyluridine(54) 2-sulfurtransferase in PDB entry 5gha, assembly 2, top view.
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Sulfur carrier protein TtuB
Chains: E, F, G, H
Length: 85 amino acids
Theoretical weight: 9.54 KDa
Source organism: Thermus thermophilus HB27
Expression system: Escherichia coli
UniProt:
  • Canonical: Q72LF4 (Residues: 1-64; Coverage: 99%)
Gene names: TT_C0105, ttuB
Pfam: ThiS family
InterPro:
Sulfur carrier protein TtuB in PDB entry 5gha, assembly 2, front view.
Sulfur carrier protein TtuB in PDB entry 5gha, assembly 2, side view.
Sulfur carrier protein TtuB in PDB entry 5gha, assembly 2, top view.
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