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Crystal structure of CoXyl43, GH43 beta-xylosidase/alpha-arabinofuranosidase from a compostmicrobial metagenome in complex with l-arabinose and xylotriose, calcium-bound form

X-ray diffraction
1.7Å resolution
PDB entry 5glr coloured by chain, front view.
PDB entry 5glr coloured by chain, side view.
PDB entry 5glr coloured by chain, top view.
Source organism: uncultured bacterium
Assembly composition:
monomeric (preferred)
Entry contents: 1 distinct polypeptide molecule

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Assemblies

Assembly 1 (preferred)
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Assembly Name: Glycoside hydrolase family 43
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Multimeric state: monomeric
Accessible surface area: 12913.21 Å2
Buried surface area: 1440.21 Å2
Dissociation area: 152.04 Å2
Dissociation energy (ΔGdiss): -5.91 kcal/mol
Dissociation entropy (TΔSdiss): 2.03 kcal/mol
Symmetry number: 1
PDBe Complex ID: PDB-CPX-102201
Assembly 2
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Assembly Name: Glycoside hydrolase family 43
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Multimeric state: monomeric
Accessible surface area: 12807.67 Å2
Buried surface area: 1234.0 Å2
Dissociation area: 408.34 Å2
Dissociation energy (ΔGdiss): -2.89 kcal/mol
Dissociation entropy (TΔSdiss): 4.97 kcal/mol
Symmetry number: 1
PDBe Complex ID: PDB-CPX-102201

Macromolecules

Glycoside hydrolase family 43
Chains: A, B
Length: 344 amino acids
Theoretical weight: 38.53 KDa
Source organism: uncultured bacterium
Expression system: Escherichia coli BL21(DE3)
UniProt:
  • Canonical: A0A0H5BL38 (Residues: 47-369; Coverage: 88%)
Gene name: coxyl43
Pfam: Glycosyl hydrolases family 43
InterPro:
CATH: Glycosyl hydrolase domain; family 43
Glycoside hydrolase family 43 in PDB entry 5glr, assembly 1, front view.
Glycoside hydrolase family 43 in PDB entry 5glr, assembly 1, side view.
Glycoside hydrolase family 43 in PDB entry 5glr, assembly 1, top view.
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