5h7b › Citations

Construction of novel repeat proteins with rigid and predictable structures using a shared helix method.

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Youn SJ, Kwon NY, Lee JH, Kim JH, Choi J, Lee H, Lee JO
OpenAccess logo Sci Rep 7 2595 (2017)
Related entries: 5h75, 5h76, 5h77, 5h78, 5h79, 5h7a, 5h7c, 5h7d, 5x3f, 5xby

Cited: 12 times
EuropePMC logo PMID: 28572639

Abstract

Generating artificial protein assemblies with complex shapes requires a method for connecting protein components with stable and predictable structures. Currently available methods for creating rigid protein assemblies rely on either complicated calculations or extensive trial and error. We describe a simple and efficient method for connecting two proteins via a fused alpha helix that is formed by joining two preexisting helices into a single extended helix. Because the end-to-end ligation of helices does not guarantee the formation of a continuous helix, we superimposed 1-2 turns of pairs of connecting helices by using a molecular graphics program. Then, we chose amino acids from the two natural sequences that would stabilize the connecting helix. This "shared helix method" is highly efficient. All the designed proteins that could be produced in Escherichia coli were readily crystallized and had the expected fusion structures. To prove the usefulness of this method, we produced two novel repeat proteins by assembling several copies of natural or artificial proteins with alpha helices at both termini. Their crystal structures demonstrated the successful assembly of the repeating units with the intended curved shapes. We propose that this method could dramatically expand the available repertoire of natural repeat proteins.

Articles - 5h7b mentioned but not cited (2)

  1. Novel structure of the N-terminal helical domain of BibA, a group B streptococcus immunogenic bacterial adhesin. Manne K, Chattopadhyay D, Agarwal V, Blom AM, Khare B, Chakravarthy S, Chang C, Ton-That H, Narayana SVL. Acta Crystallogr D Struct Biol 76 759-770 (2020)
  2. Successive remodeling of IgG glycans using a solid-phase enzymatic platform. Hsu YP, Verma D, Sun S, McGregor C, Mangion I, Mann BF. Commun Biol 5 328 (2022)


Reviews citing this publication (1)

  1. Development of imaging scaffolds for cryo-electron microscopy. Yeates TO, Agdanowski MP, Liu Y. Curr Opin Struct Biol 60 142-149 (2020)

Articles citing this publication (9)

  1. Cryo-EM structure determination of small proteins by nanobody-binding scaffolds (Legobodies). Wu X, Rapoport TA. Proc Natl Acad Sci U S A 118 e2115001118 (2021)
  2. Design of multi-scale protein complexes by hierarchical building block fusion. Hsia Y, Mout R, Sheffler W, Edman NI, Vulovic I, Park YJ, Redler RL, Bick MJ, Bera AK, Courbet A, Kang A, Brunette TJ, Nattermann U, Tsai E, Saleem A, Chow CM, Ekiert D, Bhabha G, Veesler D, Baker D. Nat Commun 12 2294 (2021)
  3. Allosteric cooperation in a de novo-designed two-domain protein. Pirro F, Schmidt N, Lincoff J, Widel ZX, Polizzi NF, Liu L, Therien MJ, Grabe M, Chino M, Lombardi A, DeGrado WF. Proc Natl Acad Sci U S A 117 33246-33253 (2020)
  4. Structural basis for ligand reception by anaplastic lymphoma kinase. Li T, Stayrook SE, Tsutsui Y, Zhang J, Wang Y, Li H, Proffitt A, Krimmer SG, Ahmed M, Belliveau O, Walker IX, Mudumbi KC, Suzuki Y, Lax I, Alvarado D, Lemmon MA, Schlessinger J, Klein DE. Nature 600 148-152 (2021)
  5. A method for structure determination of GPCRs in various states. Guo Q, He B, Zhong Y, Jiao H, Ren Y, Wang Q, Ge Q, Gao Y, Liu X, Du Y, Hu H, Tao Y. Nat Chem Biol (2023)
  6. Elfin UI: A Graphical Interface for Protein Design With Modular Building Blocks. Yeh CT, Obendorf L, Parmeggiani F. Front Bioeng Biotechnol 8 568318 (2020)
  7. Exploring rigid-backbone protein docking in biologics discovery: a test using the DARPin scaffold. Gaudreault F, Baardsnes J, Martynova Y, Dachon A, Hogues H, Corbeil CR, Purisima EO, Arbour M, Sulea T. Front Mol Biosci 10 1253689 (2023)
  8. Functionalisation of Inorganic Material Surfaces with Staphylococcus Protein A: A Molecular Dynamics Study. Farouq MAH, Kubiak-Ossowska K, Al Qaraghuli MM, Ferro VA, Mulheran PA. Int J Mol Sci 23 4832 (2022)
  9. The New Functional Hybrid Chaperone Protein ADGroEL-SacSm. Mikhaylina A, Lekontseva N, Marchenkov V, Kolesnikova V, Khairetdinova A, Nikonov O, Balobanov V. Molecules 28 6196 (2023)


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