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Crystal structure of the YgjG-protein A-Zpa963-calmodulin complex

X-ray diffraction
2.57Å resolution
PDB entry 5h7d coloured by chain, front view.
PDB entry 5h7d coloured by chain, side view.
PDB entry 5h7d coloured by chain, top view.
Source organisms:
Assembly composition:
hetero octamer (preferred)
Entry contents: 2 distinct polypeptide molecules

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Assemblies

Assembly 1 (preferred)
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Assembly Name: Immunoglobulin G-binding protein A, Putrescine aminotransferase and Calmodulin
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Multimeric state: hetero octamer
Accessible surface area: 88020.6 Å2
Buried surface area: 34128.89 Å2
Dissociation area: 4,257.59 Å2
Dissociation energy (ΔGdiss): -6.26 kcal/mol
Dissociation entropy (TΔSdiss): 66.21 kcal/mol
Symmetry number: 2
PDBe Complex ID: PDB-CPX-238624
Assembly 2
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Assembly Name: Immunoglobulin G-binding protein A, Putrescine aminotransferase and Calmodulin
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Multimeric state: hetero octamer
Accessible surface area: 88282.86 Å2
Buried surface area: 34134.22 Å2
Dissociation area: 4,270.8 Å2
Dissociation energy (ΔGdiss): -9.11 kcal/mol
Dissociation entropy (TΔSdiss): 66.22 kcal/mol
Symmetry number: 2
PDBe Complex ID: PDB-CPX-238624

Macromolecules

Immunoglobulin G-binding protein A; Putrescine aminotransferase
Chains: A, B, C, D, I, J, M, N
Length: 499 amino acids
Theoretical weight: 54.23 KDa
Source organisms: Expression system: Escherichia coli
UniProt:
  • Canonical: P42588 (Residues: 7-453; Coverage: 97%)
  • Canonical: P38507 (Residues: 220-267; Coverage: 10%)
    • nullnull
Gene names: JW5510, b3073, patA, spa, ygjG
Pfam:
InterPro:
Immunoglobulin G-binding protein A in PDB entry 5h7d, assembly 1, front view.
Immunoglobulin G-binding protein A in PDB entry 5h7d, assembly 1, side view.
Immunoglobulin G-binding protein A in PDB entry 5h7d, assembly 1, top view.
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Calmodulin
Chains: E, F, G, H, K, L, O, P
Length: 120 amino acids
Theoretical weight: 13.26 KDa
Source organism: synthetic construct
Expression system: Escherichia coli
UniProt:
  • Canonical: O16305 (Residues: 1-75; Coverage: 50%)
Gene names: T21H3.3, cmd-1
Pfam: EF-hand domain pair
InterPro:
Calmodulin in PDB entry 5h7d, assembly 1, front view.
Calmodulin in PDB entry 5h7d, assembly 1, side view.
Calmodulin in PDB entry 5h7d, assembly 1, top view.
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