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Backbone Modifications in the Protein GB1 Helix: Aib24, beta-3-Lys28, beta-3-Lys31, Aib35

X-ray diffraction
2.15Å resolution
PDB entry 5hi1 coloured by chain, front view.
PDB entry 5hi1 coloured by chain, side view.
PDB entry 5hi1 coloured by chain, top view.
Source organism: Streptococcus sp. 'group G'
Assembly composition:
monomeric (preferred)
Entry contents: 1 distinct polypeptide molecule

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Assemblies

Assembly 1
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Assembly Name: Immunoglobulin G-binding protein G
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Multimeric state: monomeric
Accessible surface area: 3643.52 Å2
Buried surface area: 106.36 Å2
Dissociation area: 53.18 Å2
Dissociation energy (ΔGdiss): 2.21 kcal/mol
Dissociation entropy (TΔSdiss): -2.55 kcal/mol
Symmetry number: 1
PDBe Complex ID: PDB-CPX-148791
Assembly 2
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Assembly Name: Immunoglobulin G-binding protein G
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Multimeric state: monomeric
Accessible surface area: 3575.92 Å2
Buried surface area: 104.78 Å2
Dissociation area: 52.39 Å2
Dissociation energy (ΔGdiss): 2.26 kcal/mol
Dissociation entropy (TΔSdiss): -2.55 kcal/mol
Symmetry number: 1
PDBe Complex ID: PDB-CPX-148791
Assembly 3 (preferred)
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Assembly Name: Immunoglobulin G-binding protein G
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Multimeric state: monomeric
Accessible surface area: 3626.45 Å2
Buried surface area: 304.98 Å2
Dissociation area: 94.07 Å2
Dissociation energy (ΔGdiss): 0.77 kcal/mol
Dissociation entropy (TΔSdiss): -0.39 kcal/mol
Symmetry number: 1
PDBe Complex ID: PDB-CPX-148791
Assembly 4
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Assembly Name: Immunoglobulin G-binding protein G
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Multimeric state: monomeric
Accessible surface area: 3744.78 Å2
Buried surface area: 219.24 Å2
Dissociation area: 51.88 Å2
Dissociation energy (ΔGdiss): -0.04 kcal/mol
Dissociation entropy (TΔSdiss): -0.39 kcal/mol
Symmetry number: 1
PDBe Complex ID: PDB-CPX-148791
Assembly 5
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Assembly Name: Immunoglobulin G-binding protein G
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Multimeric state: monomeric
Accessible surface area: 3708.9 Å2
Buried surface area: 102.92 Å2
Dissociation area: 51.46 Å2
Dissociation energy (ΔGdiss): 2.3 kcal/mol
Dissociation entropy (TΔSdiss): -2.55 kcal/mol
Symmetry number: 1
PDBe Complex ID: PDB-CPX-148791
Assembly 6
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Assembly Name: Immunoglobulin G-binding protein G
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Multimeric state: monomeric
Accessible surface area: 3614.63 Å2
Buried surface area: 116.7 Å2
Dissociation area: 58.35 Å2
Dissociation energy (ΔGdiss): 1.84 kcal/mol
Dissociation entropy (TΔSdiss): -2.54 kcal/mol
Symmetry number: 1
PDBe Complex ID: PDB-CPX-148791
Assembly 7
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Assembly Name: Immunoglobulin G-binding protein G
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Multimeric state: monomeric
Accessible surface area: 3797.48 Å2
Buried surface area: 111.67 Å2
Dissociation area: 55.83 Å2
Dissociation energy (ΔGdiss): 1.88 kcal/mol
Dissociation entropy (TΔSdiss): -2.55 kcal/mol
Symmetry number: 1
PDBe Complex ID: PDB-CPX-148791
Assembly 8
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Assembly Name: Immunoglobulin G-binding protein G
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Multimeric state: monomeric
Accessible surface area: 3644.78 Å2
Buried surface area: 115.35 Å2
Dissociation area: 57.67 Å2
Dissociation energy (ΔGdiss): 1.9 kcal/mol
Dissociation entropy (TΔSdiss): -2.54 kcal/mol
Symmetry number: 1
PDBe Complex ID: PDB-CPX-148791

Macromolecules

Immunoglobulin G-binding protein G
Chains: A, B, C, D, E, F, G, H
Length: 57 amino acids
Theoretical weight: 6.19 KDa
Source organism: Streptococcus sp. 'group G'
Expression system: Not provided
UniProt:
  • Canonical: P19909 (Residues: 302-357; Coverage: 10%)
Gene name: spg
Pfam: B domain
InterPro: IgG-binding B
CATH: Ubiquitin-like (UB roll)
Immunoglobulin G-binding protein G in PDB entry 5hi1, assembly 3, front view.
Immunoglobulin G-binding protein G in PDB entry 5hi1, assembly 3, side view.
Immunoglobulin G-binding protein G in PDB entry 5hi1, assembly 3, top view.
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