› 5hi4

X-ray diffraction
1.8Å resolution

Function and Biology Details

Biochemical function:
Biological process:
Cellular component:
Sequence domains:

Structure analysis Details

Assembly composition:
hetero heptamer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-230005 (preferred)
Entry contents:
4 distinct polypeptide molecules
Macromolecules (4 distinct):
Interleukin-17A Chains: A, B
Molecule details ›
Chains: A, B
Length: 132 amino acids
Theoretical weight: 15.15 KDa
Source organism: Homo sapiens
Expression system: Escherichia coli
UniProt:
  • Canonical: Q16552 (Residues: 24-155; Coverage: 100%)
Gene names: CTLA8, IL17, IL17A
Sequence domains: Interleukin-17
Structure domains: Cystine-knot cytokines
CAT-2000 FAB heavy chain Chains: C, H
Molecule details ›
Chains: C, H
Length: 237 amino acids
Theoretical weight: 25.05 KDa
Source organism: Homo sapiens
Expression system: Homo sapiens
Structure domains: Immunoglobulins
CAT-2000 FAB light chain Chains: D, L
Molecule details ›
Chains: D, L
Length: 214 amino acids
Theoretical weight: 23.01 KDa
Source organism: Homo sapiens
Expression system: Homo sapiens
Structure domains: Immunoglobulins
synthetic IL-17A peptide inhibitor Chain: I
Molecule details ›
Chain: I
Length: 16 amino acids
Theoretical weight: 1.85 KDa
Source organism: synthetic construct
Expression system: Not provided

Ligands and Environments

1 bound ligand:
Ligand 63P 1 x 63P
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: APS BEAMLINE 17-ID
Spacegroup: P21
Unit cell:
a: 91.24Å b: 68.19Å c: 100.12Å
α: 90° β: 91.33° γ: 90°
R-values:
R R work R free
0.201 0.2 0.224
Expression systems:
  • Escherichia coli
  • Homo sapiens
  • Not provided

Quick links

Citations

3 review citations

Structure-based design and analysis of SuFEx chemical probes.
Jones et al. (2020)
2 more

7 mentions without citation

RosettaAntibodyDesign (RAbD): A general framework for computational antibody design.
Adolf-Bryfogle et al. (2018)
6 more